SV2B Polyclonal antibody

SV2B Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 14624-1-AP

Synonyms

HsT19680, KIAA0735, SV2B

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Validation Data Gallery

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  WB analysis of human brain using 14624-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14624-1-AP (SV2B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 14624-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14624-1-AP (SV2B antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 14624-1-AP

  IP result of anti-SV2B (IP:14624-1-AP, 4ug; Detection:14624-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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  IHC staining of human brain using 14624-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14624-1-AP (SV2B antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 14624-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14624-1-AP (SV2B antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: human brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

14624-1-AP targets SV2B in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SV2B fusion protein Ag6137
• Full Name: synaptic vesicle glycoprotein 2B
• Calculated Molecular Weight: 77 kDa
• Observed Molecular Weight: 77 kDa
• GenBank Accession Number: BC030011
• Gene Symbol: SV2B
• Gene ID (NCBI): 9899
• RRID: AB_10641040
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Front Neurol; Environmental Enrichment Upregulates Striatal Synaptic Vesicle-Associated Proteins and Improves Motor Function.; Authors - Suk-Young Song
mouse	WB	Neurosci Bull; Mutant Huntingtin Causes a Selective Decrease in the Expression of Synaptic Vesicle Protein 2C.; Authors - Chaohua Peng