SVCT2 Polyclonal antibody

SVCT2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27019-1-AP

Synonyms

ascorbate transporter, hSVCT2, KIAA0238, NBTL1, SLC23A2, SVCT2, YSPL2

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Validation Data Gallery

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  WB analysis using 27019-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27019-1-AP (SVCT2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 27019-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27019-1-AP (SVCT2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 27019-1-AP

  Mouse brain lysates were subjected to SDS PAGE followed by western blot with 27019-1-AP (SVCT2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 27019-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27019-1-AP (SVCT2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 27019-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27019-1-AP (SVCT2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 27019-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27019-1-AP (SVCT2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 27019-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27019-1-AP (SVCT2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 27019-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27019-1-AP (SVCT2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Caco-2 cells, Neuro-2a cells, mouse brain tissue, rat brain tissue
• Positive IHC detected in: human colon cancer tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27019-1-AP targets SVCT2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SVCT2 fusion protein Ag25504
• Full Name: solute carrier family 23 (nucleobase transporters), member 2
• Calculated Molecular Weight: 70 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC013112
• Gene Symbol: SLC23A2
• Gene ID (NCBI): 9962
• RRID: AB_2918116
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.