SVEP1 Polyclonal antibody

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  WB analysis using 31030-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31030-1-AP (SVEP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

SVEP1 Polyclonal Antibody for WB, ELISA

Cat No. 31030-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

SELOB, SEL OB, POLYDOM, CCP22, C9orf13

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Tested Applications

• Positive WB detected in: HuH-7 cells, SMMC-7721 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31030-1-AP targets SVEP1 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SVEP1 fusion protein Ag34316
• Full Name: sushi, von Willebrand factor type A, EGF and pentraxin domain containing 1
• Calculated Molecular Weight: 390 kDa
• Observed Molecular Weight: 460 kDa
• GenBank Accession Number: NM_153366
• Gene Symbol: SVEP1
• Gene ID (NCBI): 79987
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q4LDE5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SVEP1 (C9orf13), which is predicted to be located in the cell membrane, nucleus and cytoplasm. The protein is expressed in the whole epidermis, especially in the basal layer of epidermis, the upper and lower layers of basal layer and dermal fibroblasts in the upper dermis (PMID:27892606). At the same time, it is highly expressed in placenta, lung, adrenal gland and cerebellum, but weakly expressed in heart and skeletal muscle (PMID:11062057, PMID:27892606). The molecular weight of SVEP1 is 390 kDa. At the same time, it also has several isomers of 96kDa, 163kDa and 170kDa, and there are also glycosylation modifications. It is reported that the molecular weight is 460 kDa (PMID: 32371982). The protein may plays a role in initial cell attachment of stromal osteogenic cells.