SVOP Polyclonal antibody

SVOP Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 14565-1-AP

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

SLC22B4, SV2-related protein, Synaptic vesicle 2-related protein

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIF/ICC

WB analysis using 14565-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14565-1-AP (SVOP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HEK-293 using 14565-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using SVOP antibody (14565-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
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MSDS SDS

Tested Applications

Positive WB detected in	fetal human brain tissue, mouse brain tissue, rat brain tissue
Positive IF/ICC detected in	HEK-293 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14565-1-AP targets SVOP in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag6134 Product name: Recombinant human SVOP protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 261-548 aa of BC033587 Sequence: SARYDVLSGNQEKAIATLKRIATENGAPMPLGKLIISRQEDRGKMRDLFTPHFRWTTLLLWFIWFSNAFSYYGLVLLTTELFQAGDVCGISSRKKAVEAKCSLACEYLSEEDYMDLLWTTLSEFPGVLVTLWIIDRLGRKKTMALCFVIFSFCSLLLFICVGRNVLTLLLFIARAFISGGFQAAYVYTPEVYPTATRALGLGTCSGMARVGALITPFIAQVMLESSVYLTLAVYSGCCLLAALASCFLPIETKGRGLQESSHREWGQEMVGRGMHGAGVTRSNSGSQE Predict reactive species
Full Name	SV2 related protein homolog (rat)
Calculated Molecular Weight	61 kDa
Observed Molecular Weight	67 kDa
GenBank Accession Number	BC033587
Gene Symbol	SVOP
Gene ID (NCBI)	55530
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q8N4V2
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SVOP (Synaptic Vesicle 2-Related Protein) is an evolutionarily conserved, nonglycosylated transmembrane protein localized to synaptic vesicles and microvesicles in neurons and endocrine cells. Distantly related to SV2, it contains 12 transmembrane domains and shares homology with organic ion transporters. SVOP binds adenine nucleotides, particularly NAD, and is thought to contribute to synaptic vesicle function, though its precise physiological role remains to be fully elucidated. (PMID: 9801366)