SYCE2-Specific Polyclonal antibody

• Home
• Products
• Primary Antibodies
• SYCE2-Specific Polyclonal antibody (16859-1-AP)

SYCE2-Specific Polyclonal Antibody for IHC, IF/ICC, ELISA

Cat No. 16859-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, ELISA

Synonyms

SYCE2, CESC1

• 

  IHC staining of mouse testis using 16859-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16859-1-AP (SYCE2-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 16859-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SYCE2-Specific antibody (16859-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

---

Tested Applications

• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16859-1-AP targets SYCE2-Specific in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: synaptonemal complex central element protein 2
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: NM_001105578
• Gene Symbol: SYCE2
• Gene ID (NCBI): 256126
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q6PIF2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SYCE2 (synaptonemal complex central element protein 2) is a structural component of the synaptonemal complex (SC) that is essential for meiotic chromosome synapsis and homologous recombination during meiosis. SYCE2 localizes to the central element of the synaptonemal complex, where it interacts with other SC proteins, including SYCE1, SYCE3, and TEX12, to promote assembly and stabilization of the central region. Through these interactions, SYCE2 facilitates homologous chromosome pairing, meiotic crossover formation, and accurate chromosome segregation, thereby ensuring normal gametogenesis and fertility. Deficiency or mutation of SYCE2 disrupts synaptonemal complex formation, resulting in defective meiosis, germ cell apoptosis, and infertility.