Tested Applications
| Positive WB detected in | human testis tissue, DU 145 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66409-1-Ig targets SCP3 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag19330 Product name: Recombinant human SYCP3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 13-236 aa of BC062662 Sequence: GKPSVEDQFTRAYDFETEDKKDLSGSEEDVIEGKTAVIEKRRKKRSSAGVVEDMGGEVQNMLEGVGVDINKALLAKRKRLEMYTKASLKTSNQKIEHVWKTQQDQRQKLNQEYSQQFLTLFQQWDLDMQKAEEQEEKILNMFRQQQKILQQSRIVQSQRLKTIKQLYEQFIKSMEELEKNHDNLLTGAQNEFKKEMAMLQKKIMMETQQQEIASVRKSLQSMLF Predict reactive species |
| Full Name | synaptonemal complex protein 3 |
| Calculated Molecular Weight | 236 aa, 28 kDa |
| Observed Molecular Weight | 30 kDa |
| GenBank Accession Number | BC062662 |
| Gene Symbol | SYCP3 |
| Gene ID (NCBI) | 50511 |
| RRID | AB_2881781 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q8IZU3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SYCP3 is an essential structural component of the synaptonemal complex. This complex is involved in synapsis, recombination and segregation of meiotic chromosomes. SYCP3 is required for centromere pairing during meiosis in male germ cells. SYCP3 is also required for normal meiosis during spermatogenesis and male fertility. Mutations in SYCP3 are associated with azoospermia in males and susceptibility to pregnancy loss in females.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SCP3 antibody 66409-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The SCP3 Monoclonal antibody (1A11F1), catalog number 66409-1-Ig, has 2 total citations. These appear in Environmental Pollution (IF 7.2) and Cells and Development (IF 2.5). The cited research focuses on reproductive biology and spermatogenesis—one study examined the effects of polystyrene microparticle exposure on spermatogenic cell differentiation in male mice using WB, while the other investigated the role of Samd7 in spermatogenesis and male fertility. Both citations support the antibody's application in studying meiotic chromosome dynamics and germ cell development.
| Species | Application | Title |
|---|---|---|
Environ Pollut Effects of polystyrene microparticles exposures on spermatogenic cell differentiation and reproductive endpoints in male mice | ||
