Tested Applications
| Positive WB detected in | HEK-293 cells |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16958-1-AP targets SYF2 in WB, IF/ICC, RIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10656 Product name: Recombinant human SYF2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-243 aa of BC010862 Sequence: MAAIAASEVLVDSAEEGSLAAAAELAAQKREQRLRKFRELHLMRNEARKLNHQEVVEEDKRLKLPANWEAKKARLEWELKEEEKKKECAARGEDYEKVKLLEISAEDAERWERKKKRKNPDLGFSDYAAAQLRQYHRLTKQIKPDMETYERLREKHGEEFFPTSNSLLHGTHVPSTEEIDRMVIDLEKQIEKRDKYSRRRPYNDDADIDYINERNAKFNKKAERFYGKYTAEIKQNLERGTAV Predict reactive species |
| Full Name | SYF2 homolog, RNA splicing factor (S. cerevisiae) |
| Calculated Molecular Weight | 243 aa, 29 kDa |
| Observed Molecular Weight | 29 kDa |
| GenBank Accession Number | BC010862 |
| Gene Symbol | SYF2 |
| Gene ID (NCBI) | 25949 |
| RRID | AB_2197205 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O95926 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SYF2 antibody 16958-1-AP | Download protocol |
| WB protocol for SYF2 antibody 16958-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The SYF2 Polyclonal antibody (Cat# 16958-1-AP) has 1 citation. It appears in Nucleic Acids Research (IF 15), in a study on ZRANB2 and SYF2-mediated splicing programs converging on ECT2 in breast cancer cell resistance to doxorubicin. The research field encompasses RNA splicing regulation and breast cancer drug resistance, with the antibody applied in WB and RIP assays in human samples.
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res ZRANB2 and SYF2-mediated splicing programs converging on ECT2 are involved in breast cancer cell resistance to doxorubicin.
|
Reviews
What customers say
All three reviews rated this antibody 5 stars for Western Blot. Users tested it at 1:2000–1:3000 dilution across HEK293T, RKO, and mESC cells, consistently detecting a strong Syf2 band at the expected molecular weight. One user noted two lower-molecular-weight non-specific bands alongside the target. Overall, the antibody performed reliably with overnight 4°C incubation, delivering clear and specific Syf2 detection in multiple cell types.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Moritz (Verified Customer) (09-24-2025) | Worked well for Syf2 detection in HEK293T cells
 |
Aleksandr (Verified Customer) (07-19-2025) | A major band on Syf2 is detected. Antibody was incubated overnight at 4C
 |
Tsimafei (Verified Customer) (08-07-2024) | Antibody recognize a strong band of the correct size. Also two lower nolecular weight non-specific bands are visible
 |
