Symplekin Polyclonal antibody

Symplekin Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11519-1-AP

Synonyms

SPK, SYM, SYMPK, symplekin

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Validation Data Gallery

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  WB analysis of K-562 using 11519-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11519-1-AP (Symplekin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 11519-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11519-1-AP (Symplekin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 11519-1-AP

  IP result of anti-Symplekin (IP:11519-1-AP, 4ug; Detection:11519-1-AP 1:500) with K-562 cells lysate 3600ug.

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  IHC staining of human testis using 11519-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 11519-1-AP (Symplekin antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 11519-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 11519-1-AP (Symplekin antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human placenta using 11519-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 11519-1-AP (Symplekin antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 11519-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 11519-1-AP (Symplekin antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver using 11519-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 11519-1-AP (Symplekin antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 11519-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 11519-1-AP (Symplekin antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon cancer using 11519-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11519-1-AP (Symplekin antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 11519-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11519-1-AP (Symplekin antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: K-562 cells, MCF-7 cells
• Positive IP detected in: K-562 cells
• Positive IHC detected in: human testis tissue, human colon cancer tissue, human liver tissue, human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11519-1-AP targets Symplekin in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Symplekin fusion protein Ag2081
• Full Name: symplekin
• Calculated Molecular Weight: 1274 aa, 141 kDa
• Observed Molecular Weight: 150 kDa
• GenBank Accession Number: BC006567
• Gene Symbol: SYMPK
• Gene ID (NCBI): 8189
• RRID: AB_2200281
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.