Tested Applications
| Positive WB detected in | K-562 cells, MCF-7 cells |
| Positive IP detected in | K-562 cells |
| Positive IHC detected in | human testis tissue, human colon cancer tissue, human liver tissue, human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11519-1-AP targets Symplekin in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2081 Product name: Recombinant human SYMPK protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 118-478 aa of BC006567 Sequence: MTQLYKVALQWMVKSRVISELQEACWDMVSAMAGDIILLLDSDNDGIRTHAIKFVEGLIVTLSPRMADSEIPRRQEHDISLDRIPRDHPYIQYNVLWEEGKAALEQLLKFMVHPAISSINLTTALGSLANIARQRPMFMSEVIQAYETLHANLPPTLAKSQVSSVRKNLKLHLLSVLKHPASLEFQAQITTLLVDLGTPQAEIARNMPSSKDTRKRPRDDSDSTLKKMKLEPNLGEDDEDKDLEPGPSGTSKASAQISGQSDTDITAEFLQPLLTPDNVANLVLISMVYLPEAMPASFQAIYTPVESAGTEAQIKHLARLMATQMTAAGLGPGVEQTKQCKEEPKEEKVVKTESVLIKRRL Predict reactive species |
| Full Name | symplekin |
| Calculated Molecular Weight | 1274 aa, 141 kDa |
| Observed Molecular Weight | 150 kDa |
| GenBank Accession Number | BC006567 |
| Gene Symbol | Symplekin |
| Gene ID (NCBI) | 8189 |
| RRID | AB_2200281 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q92797 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Symplekin antibody 11519-1-AP | Download protocol |
| IP protocol for Symplekin antibody 11519-1-AP | Download protocol |
| WB protocol for Symplekin antibody 11519-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Symplekin Polyclonal antibody (Cat# 11519-1-AP) has 1 citation. It appears in Frontiers in Cell and Developmental Biology (2021), in a study on SYMPK's role in meiosis and alternative splicing in male germ cells. The research field encompasses cell biology, developmental biology, and RNA processing. The antibody was validated by WB and IF in mouse models.
| Species | Application | Title |
|---|---|---|
Front Cell Dev Biol SYMPK Is Required for Meiosis and Involved in Alternative Splicing in Male Germ Cells
|
Reviews
What customers say
The single review for this product reports a 5-star rating. The user tested it in Western Blot at a 1:1000 dilution on Jurkat and HeLa cells, noting that it detects the endogenous protein with high sensitivity in both cell lines. Overall, the feedback is positive, highlighting strong performance and sensitivity for WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Fabienne (Verified Customer) (09-12-2025) | Detects endogenous protein with high sensitivity in Western blot both in HeLa and Jurkat cells
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