SYNCRIP Polyclonal antibody

• Featured Product
• KD/KO Validated

SYNCRIP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 14024-1-AP

Synonyms

HNRPQ1, HNRPQ, hnRNP Q, GRY-RBP, GRY RBP

---

Validation Data Gallery

• 

  WB analysis of A431 using 14024-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 14024-1-AP (SYNCRIP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14024-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14024-1-AP (SYNCRIP antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14024-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14024-1-AP (SYNCRIP antibody) at dilution of 1:9000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 14024-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14024-1-AP (SYNCRIP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 14024-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14024-1-AP (SYNCRIP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 14024-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 14024-1-AP (SYNCRIP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A431 using 14024-1-AP

  IP result of anti-SYNCRIP (IP:14024-1-AP, 4ug; Detection:14024-1-AP 1:500) with A431 cells lysate 1200ug.

• 

  IHC staining of human colon cancer using 14024-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14024-1-AP (SYNCRIP antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 14024-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14024-1-AP (SYNCRIP antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A431 using 14024-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using SYNCRIP antibody (14024-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). DAPI (blue).

---

Tested Applications

• Positive WB detected in: A431 cells, HEK-293 cells, Jurkat cells, HeLa cells
• Positive IP detected in: A431 cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IP: See 1 publications below

Product Information

14024-1-AP targets SYNCRIP in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SYNCRIP fusion protein Ag5167
• Full Name: synaptotagmin binding, cytoplasmic RNA interacting protein
• Calculated Molecular Weight: 623 aa, 70 kDa
• Observed Molecular Weight: 64-74 kDa
• GenBank Accession Number: BC032643
• Gene Symbol: SYNCRIP
• Gene ID (NCBI): 10492
• RRID: AB_2200601
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Synaptotagmin-binding, cytoplasmic RNA-interacting protein(SYNCRIP), also identified as hnRNP Q, involves in mRNA processing mechanisms. It's component of the CRD-mediated complex that promotes MYC mRNA stability. Also it is part of the APOB mRNA editosome complex and may modulate the postranscriptional C to U RNA-editing of the APOB mRNA through either by binding to A1CF (APOBEC1 complementation factor), to APOBEC1 or to RNA itself. May be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. Interacts in vitro preferentially with poly(A) and poly(U) RNA sequences. As previously reported, the 65 and 74 kDa proteins corresponded to two isoforms of SYNCRIP produced from alternatively spliced mRNAs (PMID: 15340051).

Publications

Species	Application	Title
human	WB,IP	Nucleic Acids Res; Comprehensive analysis of the BC200 ribonucleoprotein reveals a reciprocal regulatory function with CSDE1/UNR.; Authors - Evan P Booy
mouse	WB	Sci Rep; Identification of RNA-binding proteins that partner with Lin28a to regulate Dnmt3a expression.; Authors - Silvia Parisi ; KD Validated