SYNJ1 Polyclonal antibody

SYNJ1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 24677-1-AP

Synonyms

INPP5G, KIAA0910, synaptojanin 1, SYNJ1

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Validation Data Gallery

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  WB analysis using 24677-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24677-1-AP (SYNJ1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 24677-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 24677-1-AP (SYNJ1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 24677-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 24677-1-AP (SYNJ1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 24677-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24677-1-AP (SYNJ1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human brain using 24677-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24677-1-AP (SYNJ1 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of rat skeletal muscle using 24677-1-AP

  Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 24677-1-AP (SYNJ1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat skeletal muscle using 24677-1-AP

  Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 24677-1-AP (SYNJ1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 24677-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 24677-1-AP (SYNJ1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 24677-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 24677-1-AP (SYNJ1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skeletal muscle using 24677-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 24677-1-AP (SYNJ1 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 24677-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 24677-1-AP (SYNJ1 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of NIH/3T3 using 24677-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed NIH/3T3 cells using 24677-1-AP (SYNJ1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse brain tissue, NIH/3T3 cells, rat brain tissue
• Positive IHC detected in: human brain tissue, human skeletal muscle tissue, rat brain tissue, rat skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 1 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

24677-1-AP targets SYNJ1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SYNJ1 fusion protein Ag20364
• Full Name: synaptojanin 1
• Calculated Molecular Weight: 1612 aa, 178 kDa
• Observed Molecular Weight: 140 kDa
• GenBank Accession Number: BC136603
• Gene Symbol: SYNJ1
• Gene ID (NCBI): 8867
• RRID: AB_2879668
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Synaptojanin 1, a polyphosphoinositide phosphatase, is expressed as two major alternatively spliced isoforms of 145 kDa (SJ145) and 170 kDa (SJ170), which are thought to have pleiotropic roles in endocytosis, signaling and actin function. SJ145 is highly enriched in nerve terminals where it participates in clathrin-dependent synaptic vesicle recycling. SJ170, which differs from SJ145 by the presence of a carboxy-terminal extension, is the predominant isoform in developing neurons and is expressed in a variety of tissues.(PMID:10801423).

Publications

Species	Application	Title
human	WB	Cell Chem Biol; Triplexed Affinity Reagents to Sample the Mammalian Inositol Pyrophosphate Interactome.; Authors - David Furkert
mouse	WB,CoIP	Behav Brain Res; SYNJ1 rescues motor functions in hereditary and sporadic Parkinson's disease mice by upregulating TSP-1 expression; Authors - Yueqin Tian
mouse	IHC	Ren Fail; Network pharmacology and molecular docking of endogenous active metabolites in diabetic kidney disease; Authors - Xinmiao Xie
mouse	WB,IP	Cell Signal; Identification of novel PCTAIRE-1/CDK16 substrates using a chemical genetic screen.; Authors - Saifeldin N Shehata