Multi-rAb® SYNJ2BP Multi-Recombinant antibody

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• Multi-rAb® SYNJ2BP Multi-Recombinant antibody (RMX00068)

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  WB analysis using RMX00068

  Various lysates were subjected to SDS PAGE followed by western blot with RMX00068 (ARIP2; SYNJ2BP antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using RMX00068

  Various lysates were subjected to SDS PAGE followed by western blot with RMX00068 (ARIP2; SYNJ2BP antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse liver using RMX00068

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using RMX00068 (ARIP2; SYNJ2BP antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using RMX00068

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using RMX00068 (ARIP2; SYNJ2BP antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using RMX00068

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ARIP2; SYNJ2BP antibody (RMX00068) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

SYNJ2BP Multi-Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Cat No. RMX00068

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

ARIP2, OMP25, Synaptojanin-2-binding protein

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Tested Applications

• Positive WB detected in: A549 cells, mouse kidney tissue, HEK-293 cells, SGC-7901 cells, mouse liver tissue, rat kidney tissue
• Positive IHC detected in: mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

RMX00068 targets SYNJ2BP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Multi-Recombinant
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: synaptojanin 2 binding protein
• Calculated Molecular Weight: 145 aa, 16 kDa
• Observed Molecular Weight: 16 kDa
• GenBank Accession Number: BC007704
• Gene Symbol: SYNJ2BP
• Gene ID (NCBI): 55333
• RRID: AB_3750117
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: N/A
• UNIPROT ID: P57105
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Activin, a member of the TGF-beta superfamily, plays important roles in reproductive tissues as a stimulator of follicle-stimulating hormone (FSH) secretion and inhibits the proliferation of breast cancer cells. Activin receptor-interacting protein 2 (ARIP2), also known as synaptojanin-2-binding protein (SYNJ2BP), is located in the outer membrane of mitochondria. SYNJ2BP is widely distributed in various human tissues, and has been recently identified in mouse tissues as a regulatory protein of activin signal transduction by interacting with activin type II receptors. SYNJ2BP may be a putative growth-promoting factor involved in breast tumorigenesis and tumor development. (PMID: 23284606).