SYNPO2L Polyclonal antibody

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  WB analysis using 21480-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21480-1-AP (SYNPO2L antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 21480-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 21480-1-AP (SYNPO2L antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 21480-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 21480-1-AP (SYNPO2L antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse heart using 21480-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 21480-1-AP (SYNPO2L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 21480-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 21480-1-AP (SYNPO2L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 21480-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SYNPO2L antibody (21480-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

SYNPO2L Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 21480-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Synaptopodin 2-like protein, Synaptopodin 2 like protein

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Tested Applications

• Positive WB detected in: mouse testis tissue, PC-3 cells, rat testis tissue
• Positive IHC detected in: mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 1 publications below

Product Information

21480-1-AP targets SYNPO2L in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag15683

  Product name: Recombinant human SYNPO2L protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 35-389 aa of BC142635

  Sequence: LQRAESLQEKSIKEAKTKCRTIASLLTAAPNPHSKGVLMFKKRRQRAKKYTLVSFGAAAGTGAEEEDGVPPTSESELDEEAFSDARSLTNQSDWDSPYLDMELARAGSRASEGQGSGLGGQLSEVSGRGVQLFEQQRQRADSSTQELARVEPAAMLNGEGLQSPPRAQSAPPEAAVLPPSPLPAPVASPRPFQPGGGAPTPAPSIFNRSARPFTPGLQGQRPTTTSVIFRPLAPKRANDSLGGLSPAPPPFLSSQGPTPLPSFTSGVPSHAPVSGSPSTPRSSGPVTATSSLYIPAPSRPVTPGGAPEPPAPPSAAAMTSTASIFLSAPLRPSARPEAPAPGPGAPEPPSAREQR

• Full Name: synaptopodin 2-like
• Calculated Molecular Weight: 977 aa, 102 kDa
• Observed Molecular Weight: 79 kDa
• GenBank Accession Number: BC142635
• Gene Symbol: SYNPO2L
• Gene ID (NCBI): 79933
• RRID: AB_10860260
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9H987
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SYNPO2L belongs to the synaptopodin family and is an actin-associated protein that may play a role in modulating actin-based shape. SYNPO2L is involved in the positive regulation of Rho protein signal transduction and the positive regulation of stress fiber assembly. Loss-of-function variants in the SYNPO2L gene are associated with atrial fibrillation (PMID: 33768119).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	IF	Cells; Direct Binding of Synaptopodin 2-Like Protein to Alpha-Actinin Contributes to Actin Bundle Formation in Cardiomyocytes; Authors - Hiroshi Yamada
human	WB	Int J Biol Sci; METTL16 Promotes Stability of SYNPO2L mRNA and leading to Cancer Cell Lung Metastasis by Secretion of COL10A1 and attract the Cancer-Associated Fibroblasts; Authors - Jianlong Wu ; KD Validated