SYT17 Polyclonal antibody

• Featured Product
• KD/KO Validated

SYT17 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 15413-1-AP

Synonyms

synaptotagminXVII, Synaptotagmin-17, Synaptotagmin17, Synaptotagmin XVII, Synaptotagmin 17

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Validation Data Gallery

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  WB analysis of human kidney using 15413-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 15413-1-AP (SYT17 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 15413-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15413-1-AP (SYT17 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 15413-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15413-1-AP (SYT17 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 15413-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 15413-1-AP (SYT17 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 15413-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 15413-1-AP (SYT17 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 15413-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 15413-1-AP (SYT17 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 15413-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15413-1-AP (SYT17 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 15413-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 15413-1-AP (SYT17 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 15413-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 15413-1-AP (SYT17 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 15413-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 15413-1-AP (SYT17 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 15413-1-AP

  IP result of anti-SYT17 (IP:15413-1-AP, 4ug; Detection:15413-1-AP 1:800) with mouse brain tissue lysate 6000ug.

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  IHC staining of human brain using 15413-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15413-1-AP (SYT17 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human lung cancer using 15413-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 15413-1-AP (SYT17 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 15413-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 15413-1-AP (SYT17 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 15413-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15413-1-AP (SYT17 antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HepG2 using 15413-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SYT17 antibody (15413-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

• Positive WB detected in: human kidney tissue, A375 cells, human brain tissue, human testis tissue, mouse brain tissue, mouse lung tissue, PC-3 cells
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human brain tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

15413-1-AP targets SYT17 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SYT17 fusion protein Ag7691
• Full Name: synaptotagmin XVII
• Calculated Molecular Weight: 54 kDa
• Observed Molecular Weight: 75-80 kDa
• GenBank Accession Number: BC004518
• Gene Symbol: SYT17
• Gene ID (NCBI): 51760
• RRID: AB_2199457
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SYT17, or synaptotagmin 17, is a protein that belongs to the synaptotagmin family, which is known for its role in regulating exocytosis at the plasma membrane. Reports find high levels of expression in hippocampus, particularly in pyramidal cell layers and in cultured hippocampal neurons. SYT17 is thus a multifunctional regulator of intracellular protein trafficking in excitatory hippocampal neurons, modulating both neural development and synaptic physiology. (PMID: 31387992, PMID: 36332665)

Publications

Species	Application	Title
human	IHC,IF	Int Immunol; Increased urinary exosomal SYT17 levels in chronic active antibody-mediated rejection after kidney transplantation via the IL-6 amplifier.; Authors - Yusuke Takada ; KD Validated