Sam50 Polyclonal antibody

• 

  WB analysis using 28679-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 28679-1-AP (SAMM50 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human heart using 28679-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 28679-1-AP (Sam50 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 28679-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 28679-1-AP (Sam50 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 28679-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28679-1-AP (Sam50 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 28679-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28679-1-AP (Sam50 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 28679-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Sam50 antibody (28679-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

• 

  IF Staining of HeLa using 28679-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 28679-1-AP (Sam50 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Sam50 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 28679-1-AP

Publications(6)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

SAMM50, TOB55, SAMM 50, OMP85, CGI-51

---

Tested Applications

• Positive WB detected in: A431 cells, HeLa cells, HepG2 cells, mouse heart tissur, rat heart tissue
• Positive IHC detected in: human heart tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Product Information

28679-1-AP targets Sam50 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag29152

  Product name: Recombinant human SAMM50 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-201 aa of BC007830

  Sequence: MGTVHARSLEPLPSSGPDFGGLGEEAEFVEVEPEAKQEILENKDVVVQHVHFDGLGRTKDDIIICEIGDVFKAKNLIEVMRKSHEAREKLLRLGIFRQVDVLIDTCQGDDALPNGLDVTFEVTELRRLTGSYNTMVGNNEGSMVLGLKLPNLLGRAEKVTFQFSYGTKETSYGLSFFKPRPGNFERNFSVNLYKVTGQFPW

• Full Name: sorting and assembly machinery component 50 homolog (S. cerevisiae)
• Calculated Molecular Weight: 469 aa, 52 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC007830
• Gene Symbol: Sam50
• Gene ID (NCBI): 25813
• RRID: AB_2881192
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y512
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Sam50, encoded by the SAMM50 gene, is an essential protein of the mitochondrial outer membrane (OMM). It contains a beta-barrel domain conserved from bacteria to man (PMID: 14570913). Sam50 is a part of the sorting and assembly machinery (SAM) necessary for the assembly of beta-barrel proteins in the OMM (PMID: 22252321). It is crucial for maintaining mitochondrial shape, morphology of mitochondrial cristae, and assembly of the mitochondrial respiratory chain complexes (PMID: 26587038; 22252321; 25781180). Polymorphisms in the SAMM50 gene have been associated with development and progression of nonalcoholic fatty liver disease (PMID: 23535911).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human,mouse	WB	Dev Cell; Mitochondrial CCN1 drives ferroptosis via fatty acid β-oxidation; Authors - Wanxin Guo
mouse	IF	Front Cardiovasc Med; Samm50 Promotes Hypertrophy by Regulating Pink1-Dependent Mitophagy Signaling in Neonatal Cardiomyocytes; Authors - Ran Xu
mouse	WB	Int J Biol Sci; Loss of MTX2 causes mitochondrial dysfunction, podocyte injury, nephrotic proteinuria and glomerulopathy in mice and patients; Authors - Ting Li
mouse,rat	WB,IF,IP	Cell Signal; Mitochondrial outer membrane protein Samm50 protects against hypoxia-induced cardiac injury by interacting with Shmt2; Authors - Yufei Zhou
human	WB	Cancer Res Commun; N-Myristoyltransferase Inhibition causes Mitochondrial Iron Overload and Parthanatos in TIM17A-dependent Aggressive Lung Carcinoma; Authors - Sofia Geroyska
human	WB	J Cell Sci; Regulation of mitochondrial cristae organization by Myo19, Miro1/2 and Metaxin 3; Authors - Samruddhi S Shembekar