Selenoprotein P Recombinant antibody

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  WB analysis of human milk using 85017-4-RR

  human milk were subjected to SDS PAGE followed by western blot with 85017-4-RR (Selenoprotein P antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 85017-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85017-4-RR against Human Selenoprotein P were performed. The affinity constant is 0.587 nM.

Selenoprotein P Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 85017-4-RR

Clone No.242510E7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

SEPP1, 242510E7

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Tested Applications

• Positive WB detected in: human milk

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85017-4-RR targets Selenoprotein P in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Selenoprotein P fusion protein Ag32952
• Full Name: selenoprotein P, plasma, 1
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 45-60 kDa
• GenBank Accession Number: NM_001085486.2
• Gene Symbol: Selenoprotein P
• Gene ID (NCBI): 6414
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P49908
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Selenoprotein P, also known as SEPP1, is expressed in the liver and heart and secreted into the plasma(PMID: 8421687). Selenoprotein P is implicated as an extracellular antioxidant involved in the transport of selenium to extra-hepatic tissues via apolipoprotein E receptor-2 (apoER2) and provides selenium to tissues such as brain and testis. In humans, it exists in plasma as two isoforms of approximately 50 and 60 kDa(PMID:19453253). Selenoprotein P is a glycosylated protein and deglycosylation of SEPP1 results in 45 kDa and 35 kDa isoforms(PMID: 25298198).