Mouse P-Selectin/CD62P Recombinant antibody, PBS Only

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  WB analysis of human peripheral blood leukocyte using 83947-5-RR (same clone as 83947-5-PBS)

  human peripheral blood leukocyte were subjected to SDS PAGE followed by western blot with 83947-5-RR (P-Selectin/CD62P antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83947-5-PBS in a different storage buffer formulation.

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  IHC staining of rat spleen using 83947-5-RR (same clone as 83947-5-PBS)

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 83947-5-RR (P-Selectin/CD62P antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83947-5-PBS in a different storage buffer formulation.

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  IHC staining of rat spleen using 83947-5-RR (same clone as 83947-5-PBS)

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 83947-5-RR (P-Selectin/CD62P antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83947-5-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 83947-5-RR (same clone as 83947-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83947-5-RR (P-Selectin/CD62P antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83947-5-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 83947-5-RR (same clone as 83947-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83947-5-RR (P-Selectin/CD62P antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83947-5-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 83947-5-RR (same clone as 83947-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83947-5-RR (P-Selectin/CD62P antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83947-5-PBS in a different storage buffer formulation.

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  IF Staining of human tonsillitis using 83947-5-RR (same clone as 83947-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using P-Selectin/CD62P antibody (83947-5-RR, Clone: 240766D3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83947-5-PBS in a different storage buffer formulation.

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  IF Staining of human tonsillitis using 83947-5-RR (same clone as 83947-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using P-Selectin/CD62P antibody (83947-5-RR, Clone: 240766D3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83947-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83947-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83947-5-RR against Mouse P-Selectin/CD62P were performed. The affinity constant is 7.97 nM.

P-Selectin/CD62P {ptg:Brand} Recombinant Antibody for WB, IHC, IF-P, ELISA

Cat No. 83947-5-PBS

Clone No.240766D3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

P-selectin, CD62P, 240766D3, PADGEM, P selectin

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Product Information

83947-5-PBS targets P-Selectin/CD62P in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: selectin, platelet
• Calculated Molecular Weight: 83 kDa
• Observed Molecular Weight: 140 kDa
• GenBank Accession Number: NM_011347.2
• Gene Symbol: Selp
• Gene ID (NCBI): 20344
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q01102
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

P-selectin, also known as GMP140 or CD62P, is a transmembrane glycoprotein that mediates the interaction of activated endothelial cells or platelets with leukocytes. It is an adhesion molecule involved in the pathogenesis of inflammation, thrombosis, and oncogenesis. P-selectin is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface.