Serpin B3/4 Recombinant antibody

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  WB analysis using 85807-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85807-2-RR (SERPINB3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 85807-2-RR

  Biolayer interferometry (BLl) kinetic assays of 85807-2-RR against Human Serpin B3/4 were performed. The affinity constant is 0.95 nM.

Serpin B3/4 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 85807-2-RR

Clone No.250057B3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

SERPINB3, SERPINB3/4, Protein T4 A, Protein T4-A, SCCA-1

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Tested Applications

• Positive WB detected in: HeLa cells, L02 cells, A431 cells, HaCaT cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85807-2-RR targets Serpin B3/4 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Serpin B3/4 fusion protein Ag24846
• Full Name: serpin peptidase inhibitor, clade B (ovalbumin), member 3
• Calculated Molecular Weight: 390 aa, 45 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC005224
• Gene Symbol: SERPINB3
• Gene ID (NCBI): 6317
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P29508
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Squamous cell carcinoma antigens 1 and 2 (SCCA1 and 2, SERPIN B3 and B4) are members of the ovalbumin family of serine proteinase inhibitors. SCCA1 and SCCA2 are highly homologous proteins, 91% identical at the amino acid level, and probably evolving from a common ancestor gene. The neutral form of SCCA (SCCA1, or SERPINB3) is detected in the cytoplasm of normal and some malignant squamous cells, whereas the acidic form (SCCA2, or SERPINB4) is expressed primarily in malignant cells and is the major form found in the plasma of cancer patients. SCCA2 (SERPINB4), along with SCCA1(SERPINB3), can be processed into smaller fragments that aggregate to form an autoantigen in psoriasis, probably by causing chronic inflammation. This antibody can recognize both SerpinB3 and SerpinB4.