Serpina3k Polyclonal antibody

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• Serpina3k Polyclonal antibody (55480-1-AP)

Serpina3k Polyclonal Antibody for WB, IHC, ELISA

Cat No. 55480-1-AP Copied!

Publications (13)

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Host / Isotype

Rabbit / IgG

Reactivity

mouse and More (2)

Applications

WB, IHC, IF, ELISA

Synonyms

1300001I07Rik, contrapsin, D12Rp54, MMCM2, MMSpi2, RP54, Serpina3k, Spi 2, Spi2

• 

  WB analysis of mouse heart using 55480-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 55480-1-AP (Serpina3k antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse liver using 55480-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 55480-1-AP (Serpina3k antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse liver using 55480-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 55480-1-AP (Serpina3k antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse heart tissue
• Positive IHC detected in: mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

55480-1-AP targets Serpina3k in WB, IHC, IF, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: serine (or cysteine) peptidase inhibitor, clade A, member 3K
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 50-60 kDa
• GenBank Accession Number: NM_011458
• Gene Symbol: Serpina3k
• Gene ID (NCBI): 20714
• RRID: AB_2881346
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P07759
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

Anti-SERPINA3 affinity-purified antibody (Cat# 55480-1-AP) has 13 total citations. Journals include Nature Communications, Experimental & Molecular Medicine, Cell Molecular Life Science, FASEB Journal, Scientific Reports, International Journal of Molecular Science, Biomolecules, Cell Biology and Toxicology, American Journal of Physiology-Renal Physiology, Frontiers in Neurology, Physiological Reports, and Advanced Science. Research fields span kidney disease (AKI/CKD), liver fibrosis, cardiac injury, diabetes, neuroprotection, and oxidative stress.

Automatically generated from the 13 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
		Nat Commun; Serpina3k lactylation protects from cardiac ischemia reperfusion injury; Authors - Le Wang
mouse	WB,IHC	Exp Mol Med; Hepatocyte KCTD17-mediated SERPINA3 inhibition determines liver fibrosis in metabolic dysfunction-associated steatohepatitis.; Authors - Yelin Jeong
mouse	WB	Cell Mol Life Sci; SerpinA3k Deficiency Ameliorates Experimental type 2 Diabetes.; Authors - Isaac González-Soria ; KO Validated
mouse	WB	Cell Biol Toxicol; Neurorescuing effect of Cinacalcet against hypercalcemia-induced nerve injury in chronic kidney disease via TRAF2/cIAP1/KLF2/SERPINA3 signal axis.; Authors - Yaochen Cao
mouse	WB	Biomolecules; Molecular Profiling of Mouse Models of Loss or Gain of Function of the KCNT1 (Slack) Potassium Channel and Antisense Oligonucleotide Treatment; Authors - Fangxu Sun
mouse	WB,IF	Int J Mol Sci; SerpinA3K Deficiency Reduces Oxidative Stress in Acute Kidney Injury; Authors - Isaac González-Soria