Serpina3k Polyclonal antibody

Serpina3k Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse and More (2)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 55480-1-AP

Synonyms

1300001I07Rik, contrapsin, D12Rp54, MMCM2, MMSpi2, RP54, Serpina3k, Spi 2, Spi2

---

Validation Data Gallery

• 

  WB analysis of mouse heart using 55480-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 55480-1-AP (Serpina3k antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse liver using 55480-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 55480-1-AP (Serpina3k antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 55480-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 55480-1-AP (Serpina3k antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: mouse heart tissue
• Positive IHC detected in: mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 8 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

55480-1-AP targets Serpina3k in WB, IF, IHC, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: serine (or cysteine) peptidase inhibitor, clade A, member 3K
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 50-60 kDa
• GenBank Accession Number: NM_011458
• Gene Symbol: Serpina3k
• Gene ID (NCBI): 20714
• RRID: AB_2881346
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Cell Biol Toxicol; Neurorescuing effect of Cinacalcet against hypercalcemia-induced nerve injury in chronic kidney disease via TRAF2/cIAP1/KLF2/SERPINA3 signal axis.; Authors - Yaochen Cao
mouse	WB	Int J Mol Sci; Sirtuin 7 Deficiency Reduces Inflammation and Tubular Damage Induced by an Episode of Acute Kidney Injury.; Authors - Andrea Sánchez-Navarro
rat	WB	FASEB J; Transient response of serpinA3 during cellular stress.; Authors - Andrea Sánchez-Navarro
human	WB,IHC	Am J Physiol Renal Physiol; Urinary serpin-A3 is an early predictor of clinical response to therapy in patients with proliferative lupus nephritis; Authors - Miguel Ángel Martínez-Rojas
	WB,IHC	Sci Rep; SerpinA3 in the Early Recognition of Acute Kidney Injury to Chronic Kidney Disease (CKD) transition in the rat and its Potentiality in the Recognition of Patients with CKD.; Authors - Andrea Sánchez-Navarro
mouse	WB	Front Neurol; Neuroprotective Effects of Serpina3k in Traumatic Brain Injury.; Authors - Yao Jing