Serpinc1 Recombinant monoclonal antibody

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  WB analysis using 86029-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86029-1-RR (Serpinc1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Serpinc1 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 86029-1-RR

Clone No.250597D2

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, ELISA

Synonyms

Antithrombin-III, At3, ATIII, Serpin C1

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Tested Applications

• Positive WB detected in: mouse serum, rat serum, mouse liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86029-1-RR targets Serpinc1 in WB, ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg2949

  Product name: recombinant mouse Serpinc1 protein

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 33-465 aa of NM_080844.4

  Sequence: HGNPVDDICIAKPRDIPVNPLCIYRSPGKKATEEDGSEQKVPEATNRRVWELSKANSRFATNFYQHLADSKNDNDNIFLSPLSISTAFAMTKLGACNDTLKQLMEVFKFDTISEKTSDQIHFFFAKLNCRLYRKANKSSDLVSANRLFGDKSLTFNESYQDVSEVVYGAKLQPLDFKENPEQSRVTINNWVANKTEGRIKDVIPQGAINELTALVLVNTIYFKGLWKSKFSPENTRKEPFYKVDGQSCPVPMMYQEGKFKYRRVAEGTQVLELPFKGDDITMVLILPKPEKSLAKVEQELTPELLQEWLDELSETMLVVHMPRFRTEDGFSLKEQLQDMGLIDLFSPEKSQLPGIVAGGRDDLYVSDAFHKAFLEVNEEGSEAAASTSVVITGRSLNPNRVTFKANRPFLVLIREVALNTIIFMGRVANPCVN

• Full Name: serine (or cysteine) peptidase inhibitor, clade C (antithrombin), member 1
• Calculated Molecular Weight: 52 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: NM_080844.4
• Gene Symbol: Serpinc1
• Gene ID (NCBI): 11905
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P32261
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SERPINC1 is a gene encoding antithrombin, also known as antithrombin III. The main function of antithrombin is to inhibit coagulation proteases such as thrombin and factor Xa, thereby preventing excessive coagulation and maintaining normal hemostasis and blood flow. Mutations in the SERPINC1 gene can lead to antithrombin deficiency (ATD), which is an autosomal dominant genetic disorder. The expression profile/molecular weight observed is consistent with what has been described in the literature (PMID: 3609301, PMID: 25485983, PMID: 35935621).