Anti-Human Siglec-6/CD327 Rabbit Recombinant Antibody

• 

  FC experiment of human PBMCs using 98416-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human Siglec-6/CD327 Rabbit RecAb (98416-1-RR, Clone: 243088G2) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Human CD19. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 98416-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human Siglec-6/CD327 Rabbit RecAb (98416-1-RR, Clone: 243088G2) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. Lymphocytes were gated.

Siglec-6/CD327 Uni-rAb^TM Recombinant Antibody for FC

Cat No. 98416-1-RR

Clone No.243088G2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

SIGLEC6, CD327, CD33 antigen like 1, CD33 antigen-like 1, CD33L

---

Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98416-1-RR targets Siglec-6/CD327 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Siglec-6/CD327 fusion protein Eg2070
• Full Name: sialic acid binding Ig-like lectin 6
• Calculated Molecular Weight: 426 aa, 47 kDa
• GenBank Accession Number: BC035359
• Gene Symbol: SIGLEC6
• Gene ID (NCBI): 946
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O43699
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

Siglec-6, also known as CD327, originally identified on trophoblast cells of the placenta, is highly and consistently expressed on MCs from both mucosal and non-mucosal sites. Siglec-6 consists of 3 extracellular immunoglobulin (Ig) domains and 2 intracellular immunoreceptor tyrosine-based inhibition motifs (ITIMs), closely resembling the molecular structure of Siglec-3 (CD33) (PMID: 34289026). Siglec-6 was recruited to placental expression during human evolution, presumably to interact with sialylated ligands for specific negative signaling functions and/or to regulate leptin availability (PMID: 17580316). Siglec-6 is an immunoreceptor tyrosine-based inhibitory motif (ITIM)-bearing receptor selectively expressed by mast cells, making it a promising target for therapeutic intervention (PMID: 35406705).