Siglec-9 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 84683-4-RR (same clone as 84683-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84683-4-RR (Siglec-9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84683-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84683-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84683-4-RR against Human Siglec-9 were performed. The affinity constant is 0.492 nM.

Siglec-9 Uni-rAb^® Recombinant Antibody for WB, Indirect ELISA

Cat No. 84683-4-PBS

Clone No.241799E8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

SIGLEC9, Siglec 9, Sialic acid-binding Ig-like lectin 9, Protein FOAP 9, CD329

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Product Information

84683-4-PBS targets Siglec-9 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: sialic acid binding Ig-like lectin 9
• Calculated Molecular Weight: 50 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: NM_014441.3
• Gene Symbol: Siglec-9
• Gene ID (NCBI): 27180
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q9Y336-1
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Sialic acid binding Ig-like lectin 9 (Siglec-9), also known as CD329, is a member of the Siglec family of glycan-recognition proteins. Siglec-9 is a type-I transmembrane protein consisting of an N-terminal extracellular region that contains an N-terminal V-set domain and two C2-set domains, a transmembrane region, and an intracellular domain with an immunoreceptor tyrosine-based inhibitory motif (ITIM) and an ITIM-like motif (PMID: 10801862). It is expressed quite broadly among human blood leukocytes, including monocytes, neutrophils, B cells, NK cells, and minor subsets of T cells (PMID: 32322597). Siglec-9 functions as an inhibitory immune checkpoint and can be targeted to enhance therapeutic antitumor immunity (PMID: 34155121; 37460871).