Mouse Spag8 Polyclonal antibody, PBS Only

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• Mouse Spag8 Polyclonal antibody, PBS Only (30287-1-PBS)

• 

  WB analysis using 30287-1-AP (same clone as 30287-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 30287-1-AP (Spag8 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30287-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 30287-1-AP (same clone as 30287-1-PBS)

  rat testis tissue lysates were subjected to SDS PAGE followed by western blot with 30287-1-AP (Spag8 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30287-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse testis using 30287-1-AP (same clone as 30287-1-PBS)

  IP result of anti-Spag8 (IP:30287-1-AP, 4ug; Detection:30287-1-AP 1:3000) with mouse testis tissue lysate 1120 ug. This data was developed using the same antibody clone with 30287-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 30287-1-AP (same clone as 30287-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30287-1-AP (Spag8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30287-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 30287-1-AP (same clone as 30287-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30287-1-AP (Spag8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30287-1-PBS in a different storage buffer formulation.

Spag8 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 30287-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

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Product Information

30287-1-PBS targets Spag8 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag31603

  Product name: Recombinant mouse Spag8 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 240-422 aa of BC150868

  Sequence: EPCEPCEPPEAESEETLCKARPRGQCLLYNWEEERATNQLDQIPPLQDGSESYFFRHGHQGLLTTQPQSPMSSSTTQRDSYQLPRHICQPLRGKREAMLEMLLRHQICKEVQAEQEPARKLFETESVTHHDYRVELVRAAPPASTKPHDYRQEQPETFWIQRAARLPGVSDIRTLDTPFRKNC

• Full Name: sperm associated antigen 8
• Observed Molecular Weight: 60-70 kDa
• GenBank Accession Number: BC150868
• Gene Symbol: Spag8
• Gene ID (NCBI): 433700
• RRID: AB_3086286
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q3V0Q6
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.