ChromoTek Spot-Cap™ and Peptide

Spot-Cap® is a Nanobody-based affinity resin optimized for one-step protein purification of Spot-tagged proteins.

Specificity

Spot-Tag

Applications

Protein purification

Conjugate

Agarose

Cat no : eca-ep

Synonyms

Spot Affinity Gel; Spot Affinity Matrix



Product Information

Spot-Cap® is a Nanobody-based affinity resin optimized for one-step protein purification of Spot-tagged proteins.

ApplicationsProtein purification
Clonality Monoclonal
HostAlpaca
Specificity/TargetSpot-Tag PDRVRAVSHWSS
Coupled VHHanti-Spot-Tag single domain antibody (sdAb) fragment
ConjugateAgarose
Beads~ 90 µm (cross-linked 4 % agarose beads)
Binding capacity10 mg protein (30 kDa) / 1 mL settled resin (340 nmol / 1 mL settled resin)
Elution buffer0.1 mM Spot-peptide or 100 mM glycine pH 2.0 at 4°C
Storage ConditionShipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Shipped at ambient temperature. Upon receipt store at -20°C. Aliquot upon reconstitution and store at -20°C. Avoid repeated freeze / thaw cycles