ChromoTek Spot-Cap™ and Peptide
Spot-Cap® is a Nanobody-based affinity resin optimized for one-step protein purification of Spot-tagged proteins.
Cat no : eca-ep
Synonyms
Validation Data Gallery
Product Information
Spot-Cap® is a Nanobody-based affinity resin optimized for one-step protein purification of Spot-tagged proteins.
Applications | Protein purification |
Clonality | Monoclonal |
Host | Alpaca |
Specificity/Target | Spot-Tag PDRVRAVSHWSS |
Coupled VHH | anti-Spot-Tag single domain antibody (sdAb) fragment |
Conjugate | Unconjugated |
Beads | ~ 90 µm (cross-linked 4 % agarose beads) |
Binding capacity | 10 mg protein (30 kDa) / 1 mL settled resin (340 nmol / 1 mL settled resin) |
Elution buffer | 0.1 mM Spot-peptide or 100 mM glycine pH 2.0 at 4°C |
Storage Condition | Shipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Shipped at ambient temperature. Upon receipt store at -20°C. Aliquot upon reconstitution and store at -20°C. Avoid repeated freeze / thaw cycles |
Documentation
SDS |
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SDS Spot peptide (PDF) |
SDS Spot-Cap (PDF) |
Protocol |
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Protocol Spot-Cap (PDF) |
Datasheet |
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Spot peptide (PDF) |
Brochure |
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Spot-Cap product brochure (PDF) |