ChromoTek Spot VHH, recombinant binding protein

Validation Data Gallery

• 

  Western blot with Spot VHH.

Alpaca anti-Spot VHH, purified recombinant binding protein

Cat No. etb

Publications(5)

Specificity

Spot-Tag^®

Applications

Conjugation

Type

Nanobody

Conjugate

Unconjugated

Synonyms

Spot Nanobody, and Spot-Label (unconjugated protein)

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Product Information

Alpaca anti-Spot VHH, purified recombinant binding protein

• Description: Alpaca anti-Spot VHH, purified recombinant binding protein binds extraordinary stable & reliable to Spot-Tag®:

  • Stable up to 65 °C, 10 mM DTT, 0.75 M Guanidinium•HCl, 2 M Urea, 2 M NaCl, 2 % Nonidet P40 Substitute, 0.1 % SDS, 1 % Triton X-100, 1 % Dexoycholate

  • pH 4-10

  • Fulfills highest requirements for antibody validation

  • Structure and function are characterized

• Applications: Suitable for conjugation of dyes, biotin, beads, surfaces etc. via NHS ester reaction; Spot VHH contains a sortase-tag and a His-tag at its C terminus: LPETGHHHHHH. This sequence includes the sortase recognition motif LPETG, for conjugation. In addition, bivalent Spot-VHH may be detected by an anti-His antibody. The Spot VHH corresponds to the “BC2-Nanobody” used by Virant et al in the publication "Nature Communications" (2018) doi:10.1038/s41467-018-03191-2 refdoi:10.1038/s41467-018-03191-2
• Specificity/Target: Spot-Tag^® sequence PDRVRAVSHWSS
• Conjugate: Unconjugated
• Type: Nanobody
• Class: Recombinant
• Host: Alpaca
• Affinity (KD): Dissociation constant K_D of 6 nM
• Molecular Weight: 30.3 kDa
• RRID: AB_2827572
• Storage Buffer: 25 mM TAPS pH8,5, 500 mM NaCl, 5 mM EDTA, preservative: 0.09 % sodium azide
• Storage Condition: Shipped at ambient temperature. Upon receipt store at 4°C; stable for one year. Do not freeze.
• Spot-Tag® Origin: Engineered 12 amino acid sequence modified from the unstructured N-terminus of beta-catenin
• Cross Reactivity: The Spot-Nanobody shows minimal cross reactivity with beta-catenin.
• Size: 250 µg

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Publications

Application	Title
IF	Sci Adv; Molecular organization and mechanics of single vimentin filaments revealed by super-resolution imaging.; Authors - Filipe Nunes Vicente
WB	iScience; ProtSeq: Toward high-throughput, single-molecule protein sequencing via amino acid conversion into DNA barcodes.; Authors - Jessica M Hong
	Cells; Cep192, a Novel Missing Link between the Centrosomal Core and Corona in Dictyostelium Amoebae.; Authors - Valentin Pitzen
WB	Cells; The Phosphoarginine Phosphatase PtpB from Staphylococcus aureus Is Involved in Bacterial Stress Adaptation during Infection.; Authors - Mohamed Ibrahem Elhawy
	Life Sci Alliance; The stabilized Pol31-Pol3 interface counteracts Pol32 ablation with differential effects on repair.; Authors - Kenji Shimada