Cat no : 28a5
Mouse monoclonal antibody to Spot-tag®
|Description||Spot-tag® mouse monoclonal [28A5] for WB, ELISA|
|Specificity/Target||Spot-Tag® (PDRVRAVSHWSS) at the N-terminus, C-terminus, or internal site of the fusion protein|
|Recommended Dilution||WB: 1:1,000 - 1:5,000 |
ELISA: 1:5,000 - 1:10,000
|Immunogen||Spot-tagged fusion protein|
|Purification Method||Protein A purification|
|Storage Buffer||PBS, 50% glycerol|
|Storage Condition||Shipped on ice; Aliquot upon receipt and store at -20°C/-4°F. Avoid freeze-thaw cycles.|
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Tsimafei (Verified Customer) (06-01-2022)
mESCs express HA-SPOT-GFP protein. 20µg of total protein extract were loaded on the gel. Membrane was probed with anti-SPOT antibodies (1:1000 dilution in 3% milk) at 4C, ON.
Daniela (Verified Customer) (04-11-2022)
The 28A5 Spot-antibody was tested in Western blots of Expi-293F supernatants and purified proteins in a 1:5000 dilution using 5% non-fat milk (Biozym)/TBST (or 1x Rotiblock solution (Roth). Incubation was either 2 h at RT or ON at 4°C. Secondary HRP-antibody was incubated for 1 h at RT. The antibody is working fine, monomers, dimers, oligomers of the construct can be distinguished.