ChromoTek Spot-tag® antibody (28A5)

Mouse monoclonal antibody to Spot-tag®

Host/IsoType

Mouse IgG1

Specificity

Spot-tag®

Applications

WB, ELISA, IF (ICC)

Conjugate

Unconjugated

CloneNo.

28a5

Cat no : 28a5

Synonyms

Spot tag,Spot



Product Information

Mouse monoclonal antibody to Spot-tag®

ApplicationsWB, ELISA, IF (ICC)
Recommended DilutionWB: 1:1,000 - 1:5,000
ELISA: 1:5,000 - 1:10,000
IF (ICC): 1:200 - 1:1,000
ClonalityMonoclonal
Host/IsoTypeMouse IgG1
Specificity/TargetSpot-tag® (PDRVRAVSHWSS)
RRIDAB_2892245
ConjugateUnconjugated
CloneNo.28a5
Concentration1000 µg/mL
Storage BufferPBS, 50% glycerol, preservative: 0.09 % sodium azide
Storage ConditionShipped on blue ice. Aliquot upon receipt and store at -20°C/-4°F. Avoid freeze-thaw cycles.

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Tsimafei (Verified Customer) (06-01-2022)

mESCs expressed HA-SPOT-GFP protein (at a low level). IP was performed using in-house BC2 magnetic beads, followed by western blot. Membrane was probed with anti-SPOT antibodies at 4C, ON.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: mESCs
FH

Daniela (Verified Customer) (04-11-2022)

The 28A5 Spot-antibody was tested in Western blots of Expi-293F supernatants and purified proteins in a 1:5000 dilution using 5% non-fat milk (Biozym)/TBST (or 1x Rotiblock solution (Roth). Incubation was either 2 h at RT or ON at 4°C. Secondary HRP-antibody was incubated for 1 h at RT. The antibody is working fine, monomers, dimers, oligomers of the construct can be distinguished.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: Expi293F expression cell line