ChromoTek Spot-tag® antibody (28A5)

Mouse monoclonal antibody to Spot-tag®

Host/IsoType

Mouse/IgG1

Specificity

Spot-Tag®

Applications

WB, ELISA

Conjugate

Unconjugated

Clone No.

28a5

Cat no : 28a5

Synonyms

Spot tag,Spot



Product Information

Mouse monoclonal antibody to Spot-tag®

DescriptionSpot-tag® mouse monoclonal [28A5] for WB, ELISA
ApplicationsWB, ELISA
Specificity/TargetSpot-Tag® (PDRVRAVSHWSS) at the N-terminus, C-terminus, or internal site of the fusion protein
Recommended DilutionWB: 1:1,000 - 1:5,000
ELISA: 1:5,000 - 1:10,000
Host/IsoTypeMouse/IgG1
ClassMonoclonal
TypeAntibody
ImmunogenSpot-tagged fusion protein
Clone No.28A5
ConjugateUnconjugated
FormLiquid
Purification MethodProtein A purification
RRIDAB_2892245
Storage BufferPBS, 50% glycerol
Storage ConditionShipped on ice; Aliquot upon receipt and store at -20°C/-4°F. Avoid freeze-thaw cycles.

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Tsimafei (Verified Customer) (06-01-2022)

mESCs express HA-SPOT-GFP protein. 20µg of total protein extract were loaded on the gel. Membrane was probed with anti-SPOT antibodies (1:1000 dilution in 3% milk) at 4C, ON.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: mESCs
FH

Daniela (Verified Customer) (04-11-2022)

The 28A5 Spot-antibody was tested in Western blots of Expi-293F supernatants and purified proteins in a 1:5000 dilution using 5% non-fat milk (Biozym)/TBST (or 1x Rotiblock solution (Roth). Incubation was either 2 h at RT or ON at 4°C. Secondary HRP-antibody was incubated for 1 h at RT. The antibody is working fine, monomers, dimers, oligomers of the construct can be distinguished.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: Expi293F expression cell line