Survivin Recombinant monoclonal antibody

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• Survivin Recombinant monoclonal antibody (86850-1-RR)

Survivin Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 86850-1-RR

Clone No.251227F7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

BIRC5, Baculoviral IAP repeat-containing protein 5, BIRC 5, EPR 1, IAP4

• 

  WB analysis using 86850-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86850-1-RR (Survivin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 86850-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86850-1-RR against Human Survivin were performed. The affinity constant is 1.42 nM.

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Tested Applications

• Positive WB detected in: HeLa cells, A549 cells, Ramos cells, Jurkat cells, PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86850-1-RR targets Survivin in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg4645

  Product name: Recombinant human Survivin protein

  Source: mammalian cells-derived, pHZ-KIsec-C-rFc

  Tag: C-rFc

  Domain: 1-142 aa of /

  Sequence: MGAPTLPPAWQPFLKDHRISTFKNWPFLEGCACTPERMAEAGFIHCPTENEPDLAQCFFCFKELEGWEPDDDPIEEHKKHSSGCAFLSVKKQFEELTLGEFLKLDRERAKNKIAKETNNKKKEFEETAKKVRRAIEQLAAMD

• Full Name: baculoviral IAP repeat-containing 5
• Calculated Molecular Weight: 16kDa
• Observed Molecular Weight: 16 kDa
• GenBank Accession Number: /
• Gene Symbol: Survivin
• Gene ID (NCBI): 332
• RRID: AB_3745162
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O15392-1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Survivin, also called BIRC5, is a unique member of the inhibitor of apoptosis (IAP) protein family. Survivin is a 16 kDa anti-apoptotic protein highly expressed during fetal development and cancer cell malignancy, but is completely absent in terminally differentiated cells. The differential expression of survivin in cancer versus normal tissues makes it a useful tool in cancer diagnosis and a promising therapeutic target. Survivin expression is also highly regulated by the cell cycle and is only expressed in the G2-M phase. It is known that survivin localizes to the mitotic spindle by interaction with tubulin during mitosis and may play a contributing role in regulating mitosis. Disruption of survivin-microtubule interactions results in loss of survivin's anti-apoptosis function and increased caspase-3 activity, a mechanism involved in cell death, during mitosis. It also is a direct target gene of the Wnt pathway and is upregulated by beta-catenin.