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Anti-Human Survivin Rabbit Recombinant Antibody (98624-1-RR)

Anti-Human Survivin Rabbit Recombinant Antibody

Survivin Uni-rAb^® Recombinant Antibody for IF/ICC, FC (Intra)

Cat No. 98624-1-RR

Clone No.251227G1

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Synonyms

BIRC5, Baculoviral IAP repeat-containing protein 5, BIRC 5, EPR 1, IAP4

Formulation: PBS, Azide

PBS, Azide

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Filter: AllIF/ICCFC (Intra)Affinity

IF Staining of MCF-7 using 98624-1-RR
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Survivin antibody (98624-1-RR, Clone: 251227G1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
IF Staining of A549 using 98624-1-RR
Immunofluorescent analysis of (4% PFA) fixed A549 cells using Survivin antibody (98624-1-RR, Clone: 251227G1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
FC experiment of human PBMCs using 98624-1-RR
1x10^6 human PBMCs were intracellularly stained with 0.25 ug Anti-Human Survivin Rabbit RecAb (98624-1-RR, Clone:251227G1),or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9)and PE-Conjugated Goat Anti-Rabbit IgG(H+L),Cells were then stained with CoraLite® Plus 647 Anti-Human CD14 (26IC) (CL647-65246, Clone: 26IC),Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).Cells were incubated with FC Receptor Block prior to staining.
×
1x10^6 human PBMCs were intracellularly stained with 0.25 ug Anti-Human Survivin Rabbit RecAb (98624-1-RR, Clone:251227G1),or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9)and PE-Conjugated Goat Anti-Rabbit IgG(H+L),Cells were then stained with CoraLite® Plus 647 Anti-Human CD14 (26IC) (CL647-65246, Clone: 26IC),Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).Cells were incubated with FC Receptor Block prior to staining.
FC experiment of human PBMCs using 98624-1-RR
1x10^6 human PBMCs were intracellularly stained with 0.25 ug Anti-Human Survivin Rabbit RecAb (98624-1-RR, Clone:251227G1)(red),or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9)(blue)and PE-Conjugated Goat Anti-Rabbit IgG(H+L).Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).Cells were incubated with FC Receptor Block prior to staining.
×
1x10^6 human PBMCs were intracellularly stained with 0.25 ug Anti-Human Survivin Rabbit RecAb (98624-1-RR, Clone:251227G1)(red),or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9)(blue)and PE-Conjugated Goat Anti-Rabbit IgG(H+L).Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).Cells were incubated with FC Receptor Block prior to staining.
Affinity of 98624-1-RR
Biolayer interferometry (BLl) kinetic assays of 98624-1-RR against Human Survivin were performed. The affinity constant is 3.16 nM.

Overview

Tested Applications
Product Information
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MSDS SDS

Tested Applications

Positive IF/ICC detected in	MCF-7 cells, A549 cells
Positive FC (Intra) detected in	FC receptor blocked human PBMCs

Recommended dilution

Application	Dilution
Immunofluorescence (IF)/ICC	IF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98624-1-RR targets Survivin in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Eg4645 Product name: Recombinant human Survivin protein Source: mammalian cells-derived, pHZ-KIsec-C-rFc Tag: C-rFc Domain: 1-142 aa of / Sequence: MGAPTLPPAWQPFLKDHRISTFKNWPFLEGCACTPERMAEAGFIHCPTENEPDLAQCFFCFKELEGWEPDDDPIEEHKKHSSGCAFLSVKKQFEELTLGEFLKLDRERAKNKIAKETNNKKKEFEETAKKVRRAIEQLAAMD Predict reactive species
Full Name	baculoviral IAP repeat-containing 5
Calculated Molecular Weight	16kDa
GenBank Accession Number	/
Gene Symbol	Survivin
Gene ID (NCBI)	332
RRID	AB_3746223
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	O15392-1
Storage Buffer	PBS with 0.09% sodium azide, pH 7.3.
Storage Conditions	Store at 2 - 8°C. Stable for one year after shipment.

Background Information

Survivin, also called BIRC5, is a unique member of the inhibitor of apoptosis (IAP) protein family. Survivin is a 16 kDa anti-apoptotic protein highly expressed during fetal development and cancer cell malignancy, but is completely absent in terminally differentiated cells. The differential expression of survivin in cancer versus normal tissues makes it a useful tool in cancer diagnosis and a promising therapeutic target. Survivin expression is also highly regulated by the cell cycle and is only expressed in the G2-M phase. It is known that survivin localizes to the mitotic spindle by interaction with tubulin during mitosis and may play a contributing role in regulating mitosis. Disruption of survivin-microtubule interactions results in loss of survivin's anti-apoptosis function and increased caspase-3 activity, a mechanism involved in cell death, during mitosis. It also is a direct target gene of the Wnt pathway and is upregulated by beta-catenin.