Synaptotagmin-14 Polyclonal antibody, PBS Only

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• Synaptotagmin-14 Polyclonal antibody, PBS Only (20561-1-PBS)

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  WB analysis using 20561-1-AP (same clone as 20561-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20561-1-AP (Synaptotagmin-14 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20561-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 20561-1-AP (same clone as 20561-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 20561-1-AP (Synaptotagmin-14 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20561-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 20561-1-AP (same clone as 20561-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 20561-1-AP (Synaptotagmin-14 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20561-1-PBS in a different storage buffer formulation.

Synaptotagmin-14 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 20561-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Synaptotagmin XIV, SYT14, SytXIV

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Product Information

20561-1-PBS targets Synaptotagmin-14 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: synaptotagmin XIV
• Calculated Molecular Weight: 62 kDa
• Observed Molecular Weight: 62-65 kDa
• GenBank Accession Number: NM_153262
• Gene Symbol: Synaptotagmin-14
• Gene ID (NCBI): 255928
• RRID: AB_3085607
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8NB59
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis (PMID: 8058779). Synaptotagmin-14 (SYT14) is highly expressed in brain tissues. A homozygous mutation of SYT14 is involved in the pathogenesis of spinocerebellar ataxia (SCA) (PMID: 21835308).