Syntaxin 10 Monoclonal antibody

Syntaxin 10 Monoclonal Antibody for IF, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1B1C3

Cat no : 66821-1-Ig

Synonyms

hsyn10, STX10, SYN10, syntaxin 10

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Validation Data Gallery

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  WB analysis using 66821-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66821-1-Ig (Syntaxin 10 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 66821-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Syntaxin 10 antibody (66821-1-Ig, Clone: 1B1C3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, T-47D cells
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66821-1-Ig targets Syntaxin 10 in WB, IF, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Syntaxin 10 fusion protein Ag27861
• Full Name: syntaxin 10
• Calculated Molecular Weight: 28 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC017237
• Gene Symbol: STX10
• Gene ID (NCBI): 8677
• RRID: AB_2882164
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Syntaxin 10 (STX10), also named as SYN10, belongs to the syntaxin family. Syntaxins are target membrane SNAREs(Soluble NSF attachment protein receptors) which are membrane components that determine the specificity of the docking and fusion of vesicles to target membranes.