Syntaxin 18 Monoclonal antibody

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  WB analysis using 68220-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68220-1-Ig (Syntaxin 18 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

• 

  WB analysis using 68220-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68220-1-Ig (Syntaxin 18 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig brain using 68220-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 68220-1-Ig (Syntaxin 18 antibody) at dilution of 1:9800 incubated at room temperature for 1.5 hours.

Syntaxin 18 Monoclonal Antibody for WB, ELISA

Cat No. 68220-1-Ig

Clone No.2A2C9

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig

Applications

WB, ELISA

Synonyms

2A2C9, Cell growth-inhibiting gene 9 protein, GIG9, STX18, Syntaxin-18

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Tested Applications

• Positive WB detected in: LNCaP cells, rat brain tissue, pig brain tissue, HEK-293 cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68220-1-Ig targets Syntaxin 18 in WB, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag8947

  Product name: Recombinant human Syntaxin 18 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-317 aa of BC014613

  Sequence: MAVDITLLFRASVKTVKTRNKALGVAVGGGVDGSRDELFRRSPRPKGDFSSRAREVISHIGKLRDFLLEHRKDYINAYSHTMSEYGRMTDTERDQIDQDAQIFMRTCSEAIQQLRTEAHKEIHSQQVKEHRTAVLDFIEDYLKRVCKLYSEQRAIRVKRVVDKKRLSKLEPEPNTKTRESTSSEKVSQSPSKDSEENPATEERPEKILAETQPELGTWGDGKGEDELSPEEIQMFEQENQRLIGEMNSLFDEVRQIEGRVVEISRLQEIFTEKVLQQEAEIDSIHQLVVGATENIKEGNEDIREAIKNNAGFRVWIL

• Full Name: syntaxin 18
• Calculated Molecular Weight: 335 aa, 39 kDa
• Observed Molecular Weight: 39 kDa
• GenBank Accession Number: BC014613
• Gene Symbol: Syntaxin 18
• Gene ID (NCBI): 53407
• RRID: AB_2935308
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9P2W9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Syntaxin 18 is a novel member of the syntaxin family and it is involved in ER-mediated phagocytosis by regulating the specific and direct fusion of the ER and plasma or phagosomal membranes( PMID: 16790498).