Syntaxin 4 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 86181-1-RR (same clone as 86181-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86181-1-RR (Syntaxin 4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86181-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 86181-1-RR (same clone as 86181-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 86181-1-RR (Syntaxin 4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86181-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat kidney using 86181-1-RR (same clone as 86181-1-PBS)

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 86181-1-RR (Syntaxin 4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86181-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 86181-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86181-1-RR against Human Syntaxin 4 were performed. The affinity constant is below 1 pM.

Syntaxin 4 Uni-rAb^® Recombinant Antibody for WB, Indirect ELISA

Cat No. 86181-1-PBS

Clone No.250825G3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

Renal carcinoma antigen NY-REN-31, STX4, STX4A, Syntaxin-4

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Product Information

86181-1-PBS targets Syntaxin 4 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag6973

  Product name: Recombinant human STX4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-272 aa of BC002436

  Sequence: MRDRTHELRQGDDSSDEEDKERVALVVHPGTARLGSPDEEFFHKVRTIRQTIVKLGNKVQELEKQQVTILATPLPEESMKQELQNLRDEIKQLGREIRLQLKAIEPQKEEADENYNSVNTRMRKTQHGVLSQQFVELINKCNSMQSEYREKNVERIRRQLKITNAGMVSDEELEQMLDSGQSEVFVSNILKDTQVTRQALNEISARHSEIQQLERSIRELHDIFTFLATEVEMQGEMINRIEKNILSSADYVERGQEHVKTALENQKKARKK

• Full Name: syntaxin 4
• Calculated Molecular Weight: 34 kDa
• Observed Molecular Weight: 35-37 kDa
• GenBank Accession Number: BC002436
• Gene Symbol: STX4
• Gene ID (NCBI): 6810
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q12846
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Syntaxin 4 (STX4) is a critical SNARE protein primarily localized to the plasma membrane (PM) in mammalian cells. It plays a fundamental role in vesicle trafficking and fusion, especially in processes requiring rapid, regulated exocytosis.