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Syntaxin 7 Recombinant monoclonal antibody

Syntaxin 7 Uni-rAb® Recombinant Antibody for WB, IP, ELISA

Cat No. 87915-1-RR
Clone No.260094A2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Syntaxin-7, STX7

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inMDCK cells, Raji cells, NIH/3T3 cells, mouse spleen tissue, mouse thymus, rat spleen tissue
Positive IP detected inRaji cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

87915-1-RR targets Syntaxin 7 in WB, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag2975

Product name: Recombinant human STX7 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-244 aa of BC011975

Sequence: MSYTPGVGGDPAQLAQRISSNIQKITQCSVEIQRTLNQLGTPQDSPELRQQLQQKQQYTNQLAKETDKYIKEFGSLPTTPSEQRQRKIQKDRLVAEFTTSLTNFQKVQRQAAEREKEFVARVRASSRVSGSFPEDSSKERNLVSWESQTQPQVQVQDEEITEDDLRLIHERESSIRQLEADIMDINEIFKDLGMMIHEQGDVIDSIEANVENAEVHVQQANQQLSRAADYQRKSRKTLCIIILI

Predict reactive species
Full Name syntaxin 7
Calculated Molecular Weight 261 aa, 30 kDa
Observed Molecular Weight 35-40 kDa
GenBank Accession NumberBC011975
Gene Symbol Syntaxin 7
Gene ID (NCBI) 8417
RRIDAB_3745823
Conjugate Unconjugated
FormLiquid
Purification MethodProtein A purification
UNIPROT IDO15400
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Syntaxin 7(STX7) is a SNARE protein localized to late endosomes and lysosomes, mediating vesicle fusion and intracellular trafficking. It plays essential roles in autophagy, pathogen degradation, and cholesterol transport through interactions with VAMP8 and Vti1b.

Protocols

Product Specific Protocols
IP protocol for Syntaxin 7 antibody 87915-1-RRDownload protocol
WB protocol for Syntaxin 7 antibody 87915-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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