T7 RNA polymerase Polyclonal antibody

• 

  WB analysis of Recombinant protein using 29943-1-AP

  Recombinant protein were subjected to SDS PAGE followed by western blot with 29943-1-AP (T7 RNA polymerase antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

T7 RNA polymerase Polyclonal Antibody for WB, ELISA

Cat No. 29943-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

bacteriophage t7 and More (1)

Applications

WB, ELISA

Synonyms

T7 polymerase

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Tested Applications

• Positive WB detected in: Recombinant protein

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

29943-1-AP targets T7 RNA polymerase in WB, ELISA applications and shows reactivity with bacteriophage t7 samples.

• Tested Reactivity: bacteriophage t7
• Cited Reactivity: hamster
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag32001

  Product name: Recombinant bacteriophage t7 T7 RNA polymerase protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-300 aa of NP_041960

  Sequence: MNTINIAKNDFSDIELAAIPFNTLADHYGERLAREQLALEHESYEMGEARFRKMFERQLKAGEVADNAAAKPLITTLLPKMIARINDWFEEVKAKRGKRPTAFQFLQEIKPEAVAYITIKTTLACLTSADNTTVQAVASAIGRAIEDEARFGRIRDLEAKHFKKNVEEQLNKRVGHVYKKAFMQVVEADMLSKGLLGGEAWSSWHKEDSIHVGVRCIEMLIESTGMVSLHRQNAGVVGQDSETIELAPEYAEAIATRAGALAGISPMFQPCVVPPKPWTGITGGGYWANGRRPLALVRTH

• Full Name: T7 RNA polymerase
• Calculated Molecular Weight: 99 kDa
• GenBank Accession Number: NP_041960
• Gene Symbol: T7 RNA polymerase
• Gene ID (NCBI): 1261050
• RRID: AB_2923624
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P00573
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
		Sci Adv; Discovery of diverse and high-quality mRNA capping enzymes through a language model-enabled platform; Authors - Tianze Wang
hamster	WB	Virol Sin; Efficient and robust reverse genetics system for bovine rotavirus generation and its application for antiviral screening; Authors - Song-Kang Qin
	WB	PLoS One; T7 RNA polymerase-based gene expression from a transcriptionally silent rDNA spacer in the endosymbiont-harboring trypanosomatid Angomonas deanei; Authors - Lena Kröninger