Product Information
84959-4-PBS targets T7 RNA polymerase in WB, Indirect ELISA applications and shows reactivity with bacteriophage t7 samples.
| Tested Reactivity | bacteriophage t7 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag32001 Product name: Recombinant bacteriophage t7 T7 RNA polymerase protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-300 aa of NP_041960 Sequence: MNTINIAKNDFSDIELAAIPFNTLADHYGERLAREQLALEHESYEMGEARFRKMFERQLKAGEVADNAAAKPLITTLLPKMIARINDWFEEVKAKRGKRPTAFQFLQEIKPEAVAYITIKTTLACLTSADNTTVQAVASAIGRAIEDEARFGRIRDLEAKHFKKNVEEQLNKRVGHVYKKAFMQVVEADMLSKGLLGGEAWSSWHKEDSIHVGVRCIEMLIESTGMVSLHRQNAGVVGQDSETIELAPEYAEAIATRAGALAGISPMFQPCVVPPKPWTGITGGGYWANGRRPLALVRTH Predict reactive species |
| Full Name | T7 RNA polymerase |
| Calculated Molecular Weight | 99 kDa |
| GenBank Accession Number | NP_041960 |
| Gene Symbol | T7 RNA polymerase |
| Gene ID (NCBI) | 1261050 |
| RRID | AB_3743887 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | P00573 |
| Storage Buffer | PBS only, pH 7.3. |
| Storage Conditions | Store at -80°C. |
Background Information
T7 RNA polymerase (T7 RNAP) is a single-subunit, DNA-dependent RNA polymerase encoded by bacteriophage T7, widely used for highly specific and efficient in vitro and in vivo transcription. Unlike multi-subunit bacterial RNA polymerases, T7 RNAP recognizes a short, well-defined promoter sequence (~17 bp) and initiates transcription with exceptional promoter specificity and high processivity. It is capable of producing large amounts of RNA with minimal dependence on host transcription machinery, making it a cornerstone tool in molecular biology, synthetic biology, and biotechnology. T7 RNAP is extensively utilized in systems such as the T7 expression system (e.g., pET vectors in E. coli), in vitro transcription kits, and mRNA production workflows, including vaccine development. Structurally, it shares similarity with mitochondrial RNA polymerases and functions as a robust, fast, and orthogonal transcriptional engine. Due to its efficiency and specificity, T7 RNAP remains a fundamental tool for recombinant protein expression and RNA synthesis applications.
