T7-tag Polyclonal antibody

• 

  WB analysis using 14441-1-AP

  T7-tagged fution protein(20ng) was subjected to SDS PAGE followed by western blot with 14441-1-AP (T7-tag antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

T7-tag Polyclonal Antibody for WB, ELISA

Cat No. 14441-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

recombinant protein

Applications

WB, ELISA

Synonyms

T7 tag, T7-tag

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Tested Applications

• Positive WB detected in: Recombinant protein protein

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

14441-1-AP targets T7-tag in WB, ELISA applications and shows reactivity with recombinant protein samples.

• Tested Reactivity: recombinant protein
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: T7-tag
• Calculated Molecular Weight: 1 kDa
• RRID: AB_2878060
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The antibody is developed against a synthetic peptide MASMTGGQQMG, the T7 tag, which is present in many vectors.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
	WB	Sci Adv; TRIM37 is a primate-specific E3 ligase for Huntingtin and accounts for the striatal degeneration in Huntington's disease; Authors - Yiyang Qin
	WB	J Immunother Cancer; N-terminal acetylation of transcription factor LIP induces immune therapy resistance via suppression of PD-L1 expression in non-small cell lung cancer; Authors - Xiang He