TADA3L Polyclonal antibody

TADA3L Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 10839-1-AP

Synonyms

ADA3, ADA3 homolog, ADA3 like protein, hADA3, STAF54, TADA3, TADA3L, Transcriptional adapter 3, Transcriptional adapter 3 like

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Validation Data Gallery

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  WB analysis using 10839-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10839-1-AP (TADA3L antibody) at dilution of 1:4500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 10839-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10839-1-AP (TADA3L antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10839-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10839-1-AP (TADA3L antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 10839-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10839-1-AP (TADA3L antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 10839-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10839-1-AP (TADA3L antibody) at dilution of 1:100 (under 10x lens).

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Tested Applications

• Positive WB detected in: HeLa cells, NIH/3T3 cells, human brain tissue, Jurkat cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:9000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

10839-1-AP targets TADA3L in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TADA3L fusion protein Ag1276
• Full Name: transcriptional adaptor 3 (NGG1 homolog, yeast)-like
• Calculated Molecular Weight: 41 kDa
• Observed Molecular Weight: 49 kDa
• GenBank Accession Number: BC009240
• Gene Symbol: TADA3L
• Gene ID (NCBI): 10474
• RRID: AB_513667
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TADA3 is a subunit of 2 histone acetyltransferase complexes: the ADA2A (TADA2A)-containing (ATAC) complex and the SPT3 (SUPT3H)-TAF9-GCN5 (KAT2A)/PCAF (KAT2B) acetylase (STAGA) complex. The ATAC complex is a complex with histone acetyltransferase activity on histones H3 and H4, and the PCAF complex is capable of efficiently acetylating histones in a nucleosomal context. TADA3 is also known as a coactivator for p53/TP53-dependent transcriptional activation.

Publications

Species	Application	Title
human	WB	Nat Commun; Improved in situ characterization of protein complex dynamics at scale with thermal proximity co-aggregation; Authors - Siyuan Sun