TAF11 Polyclonal antibody

TAF11 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 16114-1-AP

Synonyms

PRO2134, TAF(II)28, TAF11, TAF2I, TAFII 28, TAFII28, TFIID subunit p30 beta

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Validation Data Gallery

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  WB analysis of HepG2 using 16114-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16114-1-AP (TAF11 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16114-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16114-1-AP (TAF11 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16114-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16114-1-AP (TAF11 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 16114-1-AP

  IP result of anti-TAF11 (IP:16114-1-AP, 4ug; Detection:16114-1-AP 1:300) with HeLa cells lysate 1600 ug.

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  IHC staining of human colon cancer using 16114-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16114-1-AP (TAF11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 16114-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16114-1-AP (TAF11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 16114-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16114-1-AP (TAF11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 16114-1-AP

  Immunofluorescent analysis of HepG2 cells using 16114-1-AP (TAF11 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16114-1-AP targets TAF11 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TAF11 fusion protein Ag9039
• Full Name: TAF11 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 28kDa
• Calculated Molecular Weight: 211 aa, 23 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: BC021972
• Gene Symbol: TAF11
• Gene ID (NCBI): 6882
• RRID: AB_2878220
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TAF11, also named as Transcription initiation factor TFIID 28 kDa subunit, is a 211 amino acid protein, which Belongs to the TAF11 family. TAF11 as a core TAFII present in both of the previously described TFIID species which either lack or contain TAFII30 . The calcualted molecular weight of TAF11 is 23 kDa, but modified TAF11 is about 28kDa. ( PMID: 10336414)