TAF1B Polyclonal antibody

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  WB analysis using 12818-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12818-1-AP (TAF1B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 12818-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12818-1-AP (TAF1B antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human testis using 12818-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12818-1-AP (TAF1B antibody) at dilution of 1:200 (under 40x lens).

TAF1B Polyclonal Antibody for WB, IHC, ELISA

Cat No. 12818-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

RAF1B, RAFI63, RNA polymerase I-specific TBP-associated factor 63 kDa, SL1, TAFI63

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Tested Applications

• Positive WB detected in: HeLa cells, NIH/3T3 cells
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

12818-1-AP targets TAF1B in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3267

  Product name: Recombinant human TAF1B protein

  Source: e coli.-derived, T-HIS

  Tag: 6*His

  Domain: 1-346 aa of BC018137

  Sequence: MDLEESEEFKERCTQCAAVSWGLTDEGKYYCTSCHNVTERYQEVTNTDLIPNTQIKALNRGLKKKNNTEKGWDWYVCEGFQYILYQQAEALKNLGVGPELKNDVLHNFWKRYLQKSKQAYCKNPVYTTGRKPTVLEDNLSHSDWASEPELLSDVSCPPFLESGAESQSDIHTRKPFPVSKASQSETSVCSGSLDGVEYSQRKEKGIVKMTMPQTLAFCYLSLLWQREAITLSDLLRFVEEDHIPYINAFQHFPEQMKLYGRDRGIFGIESWPDYEDIYKKTIEVGTFLDLPRFPDITEDCYLHPNILCMKYLMEVNLPDEMHSLTCHVVKMTGMGEVDFLTFDPIA

• Full Name: TATA box binding protein (TBP)-associated factor, RNA polymerase I, B, 63kDa
• Calculated Molecular Weight: 588 aa, 69 kDa
• Observed Molecular Weight: 69 kDa
• GenBank Accession Number: BC018137
• Gene Symbol: TAF1B
• Gene ID (NCBI): 9014
• RRID: AB_10644160
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q53T94
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TAF1B is a component of the RNA polymerase I core factor complex that acts as a GTF2B/TFIIB-like factor and plays a key role in multiple steps during transcription initiation such as pre-initiation complex (PIC) assembly and postpolymerase recruitment events in polymerase I (Pol I) transcription. It binds to rDNA promoters and plays a role in Pol I recruitment as a component of the SL1/TIF-IB complex, and possibly directly through its interaction with RRN3 (PMID: 11250903; 11283244; 15970593; 21921199).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
		Nat Commun; Genome-wide analyses of non-syndromic cleft lip with palate identify 14 novel loci and genetic heterogeneity.; Authors - Yanqin Yu
human	WB	Oncol Lett; Identification of a novel FOXO3‑associated prognostic model in hepatocellular carcinoma; Authors - Songmei Guan