TAF1B Recombinant monoclonal antibody, PBS Only

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  WB analysis of HeLa using 85885-4-RR (same clone as 85885-4-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 85885-4-RR (TAF1B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85885-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85885-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85885-4-RR against Human TAF1B were performed. The affinity constant is below 1 pM.

TAF1B Uni-rAb^® Recombinant Antibody for WB, Indirect ELISA

Cat No. 85885-4-PBS

Clone No.250162H2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

RAF1B, RAFI63, RNA polymerase I-specific TBP-associated factor 63 kDa, SL1, TAFI63

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Product Information

85885-4-PBS targets TAF1B in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag38211

  Product name: Recombinant human TAF1B protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-346 aa of BC018137

  Sequence: MDLEESEEFKERCTQCAAVSWGLTDEGKYYCTSCHNVTERYQEVTNTDLIPNTQIKALNRGLKKKNNTEKGWDWYVCEGFQYILYQQAEALKNLGVGPELKNDVLHNFWKRYLQKSKQAYCKNPVYTTGRKPTVLEDNLSHSDWASEPELLSDVSCPPFLESGAESQSDIHTRKPFPVSKASQSETSVCSGSLDGVEYSQRKEKGIVKMTMPQTLAFCYLSLLWQREAITLSDLLRFVEEDHIPYINAFQHFPEQMKLYGRDRGIFGIESWPDYEDIYKKTIEVGTFLDLPRFPDITEDCYLHPNILCMKYLMEVNLPDEMHSLTCHVVKMTGMGEVDFLTFDPIA

• Full Name: TATA box binding protein (TBP)-associated factor, RNA polymerase I, B, 63kDa
• Calculated Molecular Weight: 588 aa, 69 kDa
• Observed Molecular Weight: 69 kDa
• GenBank Accession Number: BC018137
• Gene Symbol: TAF1B
• Gene ID (NCBI): 9014
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q53T94
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

TAF1B is a component of the RNA polymerase I core factor complex that acts as a GTF2B/TFIIB-like factor and plays a key role in multiple steps during transcription initiation such as pre-initiation complex (PIC) assembly and postpolymerase recruitment events in polymerase I (Pol I) transcription. It binds to rDNA promoters and plays a role in Pol I recruitment as a component of the SL1/TIF-IB complex, and possibly directly through its interaction with RRN3 (PMID: 11250903; 11283244; 15970593; 21921199).