TAF4 Polyclonal antibody

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• TAF4 Polyclonal antibody (33029-1-AP)

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  WB analysis using 33029-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 33029-1-AP (TAF4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

TAF4 Polyclonal Antibody for WB, ELISA

Cat No. 33029-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

RNA polymerase II TBP-associated factor subunit C, TAF2C, TAF2C1, TAF4A, TAFII-130

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, HepG2 cells, L02 cells, LNCaP cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

33029-1-AP targets TAF4 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag38833

  Product name: Recombinant human TAF4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 450-590 aa of NM_003185.4

  Sequence: QLPPGMVLVRSENGQLLMIPQQALAQMQAQAHAQPQTTMAPRPATPTSAPPVQISTVQAPGTPIIARQVTPTTIIKQVSQAQTTVQPSATLQRSPGVQPQLVLGGAAQTASLGTATAVQTGTPQRTVPGATTTSSAATETM

• Full Name: TAF4 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 135kDa
• Calculated Molecular Weight: 110 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: NM_003185.4
• Gene Symbol: TAF4
• Gene ID (NCBI): 6874
• RRID: AB_3742826
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: O00268
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TAF4, also named as TAF2C, TAF2C1, TAF4A, TAFII130 and TAFII135, belongs to the TAF4 family. TAF4 makes part of TFIID is a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. TAF4 potentiates transcriptional activation by the AF-2S of the retinoic acid, vitamin D3 and thyroid hormone.