Tested Applications
| Positive WB detected in | Jurkat cells, HeLa cells, MCF-7 cells |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13506-1-AP targets TAF7 in WB, IHC, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4406 Product name: Recombinant human TAF7 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-349 aa of BC032737 Sequence: MSKSKDDAPHELESQFILRLPPEYASTVRRAVQSGHVNLKDRLTIELHPDGRHGIVRVDRVPLASKLVDLPCVMESLKTIDKKTFYKTADICQMLVSTVDGDLYPPVEEPVASTDPKASKKKDKDKEKKFIWNHGITLPLKNVRKRRFRKTAKKKYIESPDVEKEVKRLLSTDAEAVSTRWEIIAEDETKEAENQGLDISSPGMSGHRQGHDSLEHDELREIFNDLSSSSEDEDETQHQDEEDINIIDTEEDLERQLQDKLNESDEQHQENEGTNQLVMGIQKQIDNMKGKLQETQDRAKRQEDLIMKVENLALKNRFQAVLDELKQKEDREKEQLSSLQEELESLLEK Predict reactive species |
| Full Name | TAF7 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 55kDa |
| Calculated Molecular Weight | 349 aa, 40 kDa |
| Observed Molecular Weight | 55 kDa |
| GenBank Accession Number | BC032737 |
| Gene Symbol | TAF7 |
| Gene ID (NCBI) | 6879 |
| RRID | AB_2271400 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q15545 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TAF7, also named as Transcription initiation factor TFIID 55 kDa subunit, is a 349 amino acid protein, which belongs to the TAF7 family. TAF7 functions as a component of the DNA-binding general transcription factor complex TFIID, a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. TAF7 is phosphorylated by CIITA. The calcualted molecular weight of TAF7 is 40 kDa, but phosphorylated TAF7 is about 55kDa. (PMID: 22711989)
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for TAF7 antibody 13506-1-AP | Download protocol |
| IP protocol for TAF7 antibody 13506-1-AP | Download protocol |
| WB protocol for TAF7 antibody 13506-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TAF7 Polyclonal antibody (Cat# 13506-1-AP) has 4 citations published in Dev Cell, iScience, Biomedicines, and Biol Direct. The associated research fields span hematopoiesis (fetal vs. adult), triple-negative breast cancer metastasis, busulfan chemotherapy effects on male germ cell dysfunction, and colorectal cancer stemness. Applications cited include WB and ChIP, with tested species covering human and mouse.
| Species | Application | Title |
|---|---|---|
iScience TAF7 directly targets SAA1 to enhance triple-negative breast cancer metastasis via phosphorylating E-cadherin and N-cadherin
| ||
Biomedicines Busulfan Chemotherapy Downregulates TAF7/TNF-α Signaling in Male Germ Cell Dysfunction | ||
Biol Direct Single-cell RNA sequencing reveals that TAF7 promotes colorectal cancer stemness by targeting SOX2. |
Reviews
What customers say
The single review for this product reports a 5-star rating from a Western Blot application. The user tested it on K562 cells at a 1:1000 dilution and noted that it worked well with an overnight incubation at 4°C. Overall, the feedback is positive, indicating reliable performance under standard WB conditions.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Roy (Verified Customer) (09-16-2026) | Worked well with an overnight incubation at 4°C
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