TAF7 Polyclonal antibody

TAF7 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13506-1-AP

Synonyms

TAF(II)55, TAF2F, TAF7, TAFII 55, TAFII55

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Validation Data Gallery

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  WB analysis using 13506-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13506-1-AP (TAF7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 13506-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13506-1-AP (TAF7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 13506-1-AP

  IP result of anti-TAF7 (IP:13506-1-AP, 4ug; Detection:13506-1-AP 1:500) with Jurkat cells lysate 1200ug.

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  IHC staining of human cervical cancer using 13506-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 13506-1-AP (TAF7 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human cervical cancer using 13506-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 13506-1-AP (TAF7 antibody) at dilution of 1:100 (under 10x lens).

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Tested Applications

• Positive WB detected in: Jurkat cells, HeLa cells, MCF-7 cells
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13506-1-AP targets TAF7 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TAF7 fusion protein Ag4406
• Full Name: TAF7 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 55kDa
• Calculated Molecular Weight: 349 aa, 40 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC032737
• Gene Symbol: TAF7
• Gene ID (NCBI): 6879
• RRID: AB_2271400
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TAF7, also named as Transcription initiation factor TFIID 55 kDa subunit, is a 349 amino acid protein, which belongs to the TAF7 family. TAF7 functions as a component of the DNA-binding general transcription factor complex TFIID, a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. TAF7 is phosphorylated by CIITA. The calcualted molecular weight of TAF7 is 40 kDa, but phosphorylated TAF7 is about 55kDa. (PMID: 22711989)