TAF9 Polyclonal antibody

• Featured Product
• KD/KO Validated

TAF9 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

5

Reviews (1)

Cat no : 10544-1-AP

Synonyms

AD 004, AK6, CGI 137, CINAP, CIP, STAF31/32, TAF2G, TAF9, TAFII 31, TAFII 32, TAFII31, TAFII32, TAFIID32

---

Validation Data Gallery

• 

  WB analysis of HeLa using 10544-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10544-1-AP (TAF9 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 10544-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 10544-1-AP (TAF9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10544-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10544-1-AP (TAF9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10544-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10544-1-AP (TAF9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10544-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10544-1-AP (TAF9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10544-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10544-1-AP (TAF9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human colon using 10544-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 10544-1-AP (TAF9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 10544-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10544-1-AP (TAF9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 10544-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 10544-1-AP (TAF9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse skeletal muscle using 10544-1-AP

  IP result of anti-TAF9 (IP:10544-1-AP, 4ug; Detection:10544-1-AP 1:1000) with mouse skeletal muscle tissue lysate 2200ug.

---

Tested Applications

• Positive WB detected in: HeLa cells, MCF-7 cells, human colon tissue, human skeletal muscle tissue, HEK-293 cells, human heart tissue, rat liver tissue
• Positive IP detected in: mouse skeletal muscle tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 2 publications below
• IP: See 1 publications below

Product Information

10544-1-AP targets TAF9 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TAF9 fusion protein Ag0833
• Full Name: TAF9 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 32kDa
• Calculated Molecular Weight: 28 kDa
• Observed Molecular Weight: 24-28 kDa
• GenBank Accession Number: BC007426
• Gene Symbol: TAF9
• Gene ID (NCBI): 6880
• RRID: AB_2271416
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TAF9, also named as TAF2G, TAFII31, is a 264 amino acid protein, which belongs to the TAF9 family. TAF9 are involved in transcriptional activation as well as repression of distinct but overlapping sets of genes. TAF9 may have a role in gene regulation associated with apoptosis.

Publications

Species	Application	Title
human	IHC	Cancers (Basel); Identification of Prognostic Markers of Gynecologic Cancers Utilizing Patient-Derived Xenograft Mouse Models.; Authors - Ha-Yeon Shin
human	WB	Front Oncol; Stable Isotope Tracing Uncovers Reduced γ/β-ATP Turnover and Metabolic Flux Through Mitochondrial-Linked Phosphotransfer Circuits in Aggressive Breast Cancer Cells.; Authors - Aleksandr Klepinin
mouse,rat	WB,IHC,IP	Front Pharmacol; Activation of TAF9 via Danshensu-Induced Upregulation of HDAC1 Expression Alleviates Non-alcoholic Fatty Liver Disease.; Authors - Ruiwen Wang ; KD Validated
human	WB	J Proteome Res; Targeted Proteomic Analysis Revealed Kinome Reprogramming during Acquisition of Radioresistance in Breast Cancer Cells.; Authors - Weili Miao
human	WB	Mol Cell Proteomics; Multi-Level Proteomics Reveals Epigenetic Signatures in BCG-mediated Macrophage Activation; Authors - Zoe Schaefer

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Aditya (Verified Customer) (10-08-2024)

good, very low on blot - convenient

• Applications: Western Blot