TAF9B Polyclonal antibody

TAF9B Polyclonal Antibody for IHC, IP,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IP, IHC,ELISA

Conjugate

Unconjugated

Cat no : 10921-1-AP

Synonyms

DN 7, DN7, TAF9B, TAF9L, TAFII31L, TFIID 31

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Validation Data Gallery

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  IP experiment of HeLa using 10921-1-AP

  IP result of anti-TAF9B (IP:10921-1-AP, 4ug; Detection:10921-1-AP 1:300) with HeLa cells lysate 4000ug.

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  IHC staining of human breast cancer using 10921-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10921-1-AP (TAF9B antibody) at dilution of 1:100 (under 10x lens).

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Tested Applications

• Positive IP detected in: HeLa cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

10921-1-AP targets TAF9B in IP, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TAF9B fusion protein Ag1362
• Full Name: TAF9B RNA polymerase II, TATA box binding protein (TBP)-associated factor, 31kDa
• Calculated Molecular Weight: 28 kDa
• Observed Molecular Weight: 26 kDa
• GenBank Accession Number: BC010350
• Gene Symbol: TAF9B
• Gene ID (NCBI): 51616
• RRID: AB_2199344
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Transcription initiation factor TFIID subunit 9B (TAF9B), also names neuronal cell death-related protein 7,transcription initiation factor TFIID subunit 9-like,transcription-associated factor TAFII31L. It is essential for cell viability. TAF9 and TAF9B are involved in transcriptional activation as well as repression of distinct but overlapping sets of genes. May have a role in gene regulation associated with apoptosis. TAFs are components of the transcription factor IID (TFIID) complex, the TBP-free TAFII complex (TFTC), the PCAF histone acetylase complex and the STAGA transcription coactivator-HAT complex. TFIID or TFTC are essential for the regulation of RNA polymerase II-mediated transcription.It's molecular mass is 31kd.