TALDO1 Polyclonal antibody

• Featured Product
• KD/KO Validated

TALDO1 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 12376-1-AP

Synonyms

transaldolase 1, TALDOR, TALDO, EC:2.2.1.2

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Validation Data Gallery

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  WB analysis using 12376-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12376-1-AP (TALDO1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 12376-1-AP

  WB result of TALDO1 antibody (12376-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TALDO1 transfected HEK-293 cells.

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  IP experiment of COLO 320 using 12376-1-AP

  IP result of anti-TALDO1 (IP:12376-1-AP, 4ug; Detection:12376-1-AP 1:700) with COLO 320 cells lysate 3200 ug.

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  IHC staining of human colon using 12376-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 12376-1-AP (TALDO1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 12376-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 12376-1-AP (TALDO1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A431 cells, HEK-293 cells, COLO 320 cells, HepG2 cells
• Positive IP detected in: COLO 320 cells
• Positive IHC detected in: human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below

Product Information

12376-1-AP targets TALDO1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TALDO1 fusion protein Ag3031
• Full Name: transaldolase 1
• Calculated Molecular Weight: 337 aa, 38 kDa
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: BC009680
• Gene Symbol: TALDO1
• Gene ID (NCBI): 6888
• RRID: AB_2199712
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TALDO1 belongs to the transaldolase family which contributes to the generation of NADPH in the nonoxidative phase of the pentose phosphate pathway (PPP). Defects in TALDO1 are the cause of transaldolase 1 deficiency which results in telangiectases of the skin, hepatosplenomegaly, and enlarged clitoris.

Publications

Species	Application	Title
		Science; mTORC1 induces purine synthesis through control of the mitochondrial tetrahydrofolate cycle.; Authors - Issam Ben-Sahra
human	WB	Cancer Cell; Nrf2 redirects glucose and glutamine into anabolic pathways in metabolic reprogramming.; Authors - Yoichiro Mitsuishi
human	WB	Nat Commun; Synthetic lethality between HER2 and transaldolase in intrinsically resistant HER2-positive breast cancers.; Authors - Yi Ding ; KD Validated
human	WB	Nat Metab; Mitochondrial NADP+ is essential for proline biosynthesis during cell growth.; Authors - Diem H Tran
		J Biol Chem; Metabolic reprogramming driven by EZH2 inhibition depends on cell-matrix interactions; Authors - Teresa W-M Fan
human	WB	Cell Rep; A pro-metastatic tRNA fragment drives aldolase A oligomerization to enhance aerobic glycolysis in lung adenocarcinoma; Authors - Qinglin Wang