Tested Applications
| Positive WB detected in | A431 cells, HEK-293 cells, COLO 320 cells, HepG2 cells |
| Positive IP detected in | COLO 320 cells |
| Positive IHC detected in | human colon tissue, mouse colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12376-1-AP targets TALDO1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3031 Product name: Recombinant human TALDO1 protein Source: e coli.-derived, T-HIS Tag: 6*His Domain: 1-277 aa of BC009680 Sequence: MSSSPVKRQRMESALDQLKQFTTVVADTGDFHAIDEYKPQDATTNPSLILAAAQMPAYQELVEEAIAYGRKLGGSQEDQIKNAIDKLFVLFGAEILKKIPGRVSTEVDARLSFDKDAMVARARRLIELYKEAGISKDRILIKLSSTWEGIQAGKELEEQHGIHCNMTLLFSFAQAVACAEAGVTLISPFVGRILDWHVANTDKKSYEPLEDPGVKSVTKIYNYYKKFSYKTIVMGASFRNTGEIKALAGCDFLTISPKLLGELLQDNAKLVPVLSAK Predict reactive species |
| Full Name | transaldolase 1 |
| Calculated Molecular Weight | 337 aa, 38 kDa |
| Observed Molecular Weight | 38 kDa |
| GenBank Accession Number | BC009680 |
| Gene Symbol | TALDO1 |
| Gene ID (NCBI) | 6888 |
| RRID | AB_2199712 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P37837 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TALDO1 belongs to the transaldolase family which contributes to the generation of NADPH in the nonoxidative phase of the pentose phosphate pathway (PPP). Defects in TALDO1 are the cause of transaldolase 1 deficiency which results in telangiectases of the skin, hepatosplenomegaly, and enlarged clitoris.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for TALDO1 antibody 12376-1-AP | Download protocol |
| IP protocol for TALDO1 antibody 12376-1-AP | Download protocol |
| WB protocol for TALDO1 antibody 12376-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TALDO1 Polyclonal antibody (Cat# 12376-1-AP) has 11 citations. These appear in journals including Cancer Cell, Science, Nature Metabolism, Nature Communications, Cell Death & Disease, Clinical and Translational Medicine, Cell Reports, Journal of Agricultural and Food Chemistry, Ecotoxicology and Environmental Safety, Food & Function, and the Journal of Biological Chemistry. Research fields span cancer metabolism, metabolic reprogramming, the pentose phosphate pathway, glycolysis, and lipid metabolism, with applications primarily in Western blot, immunoprecipitation, and immunohistochemistry.
| Species | Application | Title |
|---|---|---|
Cancer Cell Nrf2 redirects glucose and glutamine into anabolic pathways in metabolic reprogramming. | ||
Science mTORC1 induces purine synthesis through control of the mitochondrial tetrahydrofolate cycle. | ||
Nat Commun Synthetic lethality between HER2 and transaldolase in intrinsically resistant HER2-positive breast cancers.
| ||
Cell Death Dis Deacetylation of TALDO1 by HDAC6 promotes glycolysis and nasopharyngeal carcinoma progression through a moonlighting function.
| ||
Clin Transl Med TALDO1 promotes lipid metabolic reprogramming and immunosuppressive microenvironment remodelling in hepatocellular carcinoma |
