TAOK2 Polyclonal antibody

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• KD/KO Validated

TAOK2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 21188-1-AP

Synonyms

hKFC C, KIAA0881, Kinase from chicken homolog C, MAP3K17, PSK, PSK 1, PSK1, TAO kinase 2, TAO1, TAO2, TAOK2

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Validation Data Gallery

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  WB analysis of mouse brain using 21188-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21188-1-AP (TAOK2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 21188-1-AP

  WB result of TAOK2 antibody (21188-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-control and sh-TAOK2 transfected HEK-293 cells.

• 

  WB analysis of HEK-293 using 21188-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21188-1-AP (TAOK2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 21188-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21188-1-AP (TAOK2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 21188-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 21188-1-AP (TAOK2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 21188-1-AP

  IP result of anti-TAOK2 (IP:21188-1-AP, 4ug; Detection:21188-1-AP 1:500) with HEK-293 cells lysate 3000ug.

• 

  IHC staining of human liver cancer using 21188-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21188-1-AP (TAOK2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 21188-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21188-1-AP (TAOK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue, HEK-293 cells, L02 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below
• IF: See 3 publications below

Product Information

The immunogen of 21188-1-AP is TAOK2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, Monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TAOK2 fusion protein Ag15521
• Full Name: TAO kinase 2
• Calculated Molecular Weight: 1235 aa, 138 kDa
• Observed Molecular Weight: 138-150 kDa
• GenBank Accession Number: BC142663
• Gene Symbol: TAOK2
• Gene ID (NCBI): 9344
• RRID: AB_10755293
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TAOK2(Serine/threonine-protein kinase TAO2) is also named as KIAA0881, MAP3K17, PSK, PSK1 and belongs to the protein kinase superfamily. TAOK2 is known to modulate gene transcription via its activation of several MAP kinase pathways, including c-Jun amino-terminal kinase (JNK), and has been implicated in the modulation of actin and microtubule dynamics. The time course of TAOK2 expression is consistent with a role for it in neuronal differentiation.(PMID:22735514). The immunogen of this antibody is 742-969 aa of human TAOK2 protein isoform Alpha.This antibody mainly detects the isoform 1/3/4. It can't detect the isoform 2.

Publications

Species	Application	Title
human	WB,IF	Am J Hum Genet; Variants in EXOSC9 Disrupt the RNA Exosome and Result in Cerebellar Atrophy with Spinal Motor Neuronopathy.; Authors - David T Burns
human	WB,IF	Acta Neuropathol Commun; A new TAO kinase inhibitor reduces tau phosphorylation at sites associated with neurodegeneration in human tauopathies.; Authors - Caterina Giacomini
human	WB,IF	Mol Cancer Ther; Targeting TAO kinases using a new inhibitor compound delays mitosis and induces mitotic cell death in centrosome amplified breast cancer cells.; Authors - Chuay-Yeng Koo
Monkey	WB	J Biol Chem; Prostate-derived sterile 20-like kinases (PSKs/TAOKs) phosphorylate tau protein and are activated in tangle-bearing neurons in Alzheimer disease.; Authors - Tavares Ignatius A IA
human	WB	Mol Carcinog; Targeting TAOK1 with resveratrol inhibits esophageal squamous cell carcinoma growth in vitro and in vivo; Authors - Mengqiu Song
monkey	WB	J Cell Sci; Rnd3 interacts with TAO kinases and contributes to mitotic cell rounding and spindle positioning.; Authors - Ritu Garg