TAOK3 Polyclonal antibody

• Featured Product
• KD/KO Validated

TAOK3 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 10158-2-AP

Synonyms

DPK, hKFC A, JIK, JNK/SAPK inhibitory kinase, Jun kinase inhibitory kinase, KDS, Kinase from chicken homolog A, MAP3K18, TAO kinase 3, TAOK3

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Validation Data Gallery

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  WB analysis of human liver using 10158-2-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 10158-2-AP (TAOK3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 10158-2-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10158-2-AP (TAOK3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10158-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10158-2-AP (TAOK3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 10158-2-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10158-2-AP (TAOK3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 10158-2-AP

  IP result of anti-TAOK3 (IP:10158-2-AP, 4ug; Detection:10158-2-AP 1:700) with HepG2 cells lysate 3600ug.

• 

  IHC staining of human liver using 10158-2-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10158-2-AP (TAOK3 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 10158-2-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10158-2-AP (TAOK3 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human liver using 10158-2-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 10158-2-AP (TAOK3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 10158-2-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 10158-2-AP (TAOK3 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: human liver tissue, HeLa cells, HepG2 cells, human brain tissue
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IP: See 1 publications below

Product Information

10158-2-AP targets TAOK3 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TAOK3 fusion protein Ag0211
• Full Name: TAO kinase 3
• Calculated Molecular Weight: 105 kDa
• Observed Molecular Weight: 100-105 kDa
• GenBank Accession Number: BC002756
• Gene Symbol: TAOK3
• Gene ID (NCBI): 51347
• RRID: AB_2255688
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TAOK3(Serine/threonine-protein kinase TAO3) is also named as DPK, JIK, KDS, MAP3K18 and belongs to the STE Ser/Thr protein kinase family. It acts as an activator of the p38/MAPK14 stress-activated MAPK cascade. In response to DNA damage, it is involved in the G2/M transition DNA damage checkpoint by activating the p38/MAPK14 stress-activated MAPK cascade, probably by mediating phosphorylation of upstream MAP2K3 and MAP2K6 kinases. And this protein can be autophosphorylated(PMID:20949042).

Publications

Species	Application	Title
human	WB	Mol Carcinog; Targeting TAOK1 with resveratrol inhibits esophageal squamous cell carcinoma growth in vitro and in vivo; Authors - Mengqiu Song
human	WB	Cell Commun Signal; Kinase shRNA screening reveals that TAOK3 enhances microtubule-targeted drug resistance of breast cancer cells via the NF-κB signaling pathway.; Authors - Tsung-Ching Lai ; KD Validated
mouse	IP	Sci Signal; The induction of SHP-1 degradation by TAOK3 ensures the responsiveness of T cells to TCR stimulation; Authors - Alexandre Poirier