TDP-43 (full length) Polyclonal antibody

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  WB analysis using 18280-1-AP

  K562 cells were subjected to SDS PAGE followed by western blot with 18280-1-AP (TDP-43 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 18280-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 18280-1-AP (TDP-43 (full length) antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 18280-1-AP

  IP result of anti-TDP-43 (IP:18280-1-AP, 3ug; Detection:18280-1-AP 1:1500) with K-562 cells lysate 6000ug.

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  IHC staining of human gliomas using 18280-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 18280-1-AP (TDP-43 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 18280-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18280-1-AP (TDP-43 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 18280-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 18280-1-AP (TDP-43 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 18280-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 18280-1-AP (TDP-43 (full length) antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of rat brain using 18280-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 18280-1-AP (TDP-43 (full length) antibody at dilution of 1:200 (under 40x lens).

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  IF Staining of SH-SY5Y using 18280-1-AP

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using TDP-43 antibody (18280-1-AP) at dilution of 1:3000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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  FC experiment of SH-SY5Y using 18280-1-AP

  1x10^6 SH-SY5Y cells were intracellularly stained with 0.2 ug TDP-43 (full length) Polyclonal antibody (18280-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.2 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

TDP-43 (full length) Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 18280-1-AP

Publications(16)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Synonyms

TARDBP, TDP 43, TDP43, TDP-43, ALS10

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Tested Applications

• Positive WB detected in: K-562 cells
• Positive IP detected in: K-562 cells
• Positive IHC detected in: mouse brain tissue, rat brain tissue, human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: SH-SY5Y cells
• Positive FC (Intra) detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:1500-1:6000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IHC: See 6 publications below
• IF: See 5 publications below
• IP: See 2 publications below

Product Information

18280-1-AP targets TDP-43 (full length) in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: TAR DNA binding protein
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 43 kDa
• GenBank Accession Number: BC001487
• Gene Symbol: TDP-43
• Gene ID (NCBI): 23435
• RRID: AB_2240312
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q13148
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Transactivation response (TAR) DNA-binding protein of 43 kDa (also known as TARDBP or TDP-43) was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterize amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). This antibody recognizes the cleavage product of 20-30 kDa in addition to the native and phosphorylated forms of TDP-43. Immunohistochemical analyses of TDP-43 using this antibody detect both normal diffuse nuclear staining and insoluble inclusions in pathologic tissues.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
		Nat Neurosci; TDP-43 extracted from frontotemporal lobar degeneration subject brains displays distinct aggregate assemblies and neurotoxic effects reflecting disease progression rates.; Authors - Florent Laferrière
human	IHC	Acta Neuropathol Commun; Anatomic survey of seeding in Alzheimer's disease brains reveals unexpected patterns.; Authors - Barbara E Stopschinski
human	WB	Neurobiol Dis; Tau-tubulin kinase 1 phosphorylates TDP-43 at disease-relevant sites and exacerbates TDP-43 pathology; Authors - Yuan Tian
mouse	IF	Dis Model Mech; Viral delivery of C9ORF72 hexanucleotide repeat expansions in mice lead to repeat length dependent neuropathology and behavioral deficits.; Authors - Saul Herranz-Martin
human	IHC	J Neurotrauma; Multimodal Biomarkers of Repetitive Head Impacts and Traumatic Encephalopathy Syndrome: A Clinico-Pathological Case Series.; Authors - Breton Asken
human	IHC	J Alzheimers Dis; Pathological Correlates of Cognitive Impairment in The University of Manchester Longitudinal Study of Cognition in Normal Healthy Old Age.; Authors - Andrew C Robinson