TARS Polyclonal antibody

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  WB analysis of A549 using 14773-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 14773-1-AP (TARS antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 14773-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 14773-1-AP (TARS antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of A549 using 14773-1-AP

  IP result of anti-TARS (IP:14773-1-AP, 4ug; Detection:14773-1-AP 1:500) with A549 cells lysate 2400ug.

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  IHC staining of human breast cancer using 14773-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14773-1-AP (TARS Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 14773-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14773-1-AP (TARS Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 14773-1-AP

  Immunofluorescent analysis of MCF-7 cells, using TARS antibody 14773-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  IF Staining of HepG2 using 14773-1-AP

  Immunofluorescent analysis of HepG2 cells, using TARS antibody 14773-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  IF Staining of HeLa using 14773-1-AP

  Immunofluorescent analysis of Hela cells, using TARS antibody 14773-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

TARS Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 14773-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Threonyl-tRNA synthetase 1, Threonyl-tRNA synthetase, Threonine--tRNA ligase 1, cytoplasmic, Threonine tRNA ligase, TARS1

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Tested Applications

• Positive WB detected in: A549 cells
• Positive IP detected in: A549 cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells, Hela cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IP: See 1 publications below

Product Information

14773-1-AP targets TARS in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6614

  Product name: Recombinant human TARS protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 394-723 aa of BC000517

  Sequence: ENMFSFEVEKELFALKPMNCPGHCLMFDHRPRSWRELPLRLADFGVLHRNELSGALTGLTRVRRFQQDDAHIFCAMEQIEDEIKGCLDFLRTVYSVFGFSFKLNLSTRPEKFLGDIEVWDQAEKQLENSLNEFGEKWELNSGDGAFYGPKIDIQIKDAIGRYHQCATIQLDFQLPIRFNLTYVSHDGDDKKRPVIVHRAILGSVERMIAILTENYGGKWPFWLSPRQVMVVPVGPTCDEYAQKVRQQFHDAKFMADIDLDPGCTLNKKIRNAQLAQYNFILVVGEKEKISGTVNIRTRDNKVHGERTISETIERLQQLKEFRSKQAEEEF

• Full Name: threonyl-tRNA synthetase
• Calculated Molecular Weight: 83 kDa
• Observed Molecular Weight: 66-72 kDa
• GenBank Accession Number: BC000517
• Gene Symbol: TARS
• Gene ID (NCBI): 6897
• RRID: AB_2200654
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P26639
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TARS (Threonyl-tRNA synthetase), also known as threonine-tRNA ligase or ThrRS, belongs to the aminoacyl-tRNA synthetase family. Aminoacyl-tRNA synthetase (aaRS) catalyzes the specific esterification of an amino acid with the 3'-terminal hydroxyl group of its cognate tRNA. Specificity of aminoacylation catalyzed by aaRSs ensures the accurate translation of the genetic code in the first step of protein synthesis (PMID: 26324710). TARS has some isoforms with the molecular mass of 83, 87 kDa and other MW.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Mol Cell; Mitochondrial Threonyl-tRNA Synthetase TARS2 Is Required for Threonine-Sensitive mTORC1 Activation.; Authors - Sung-Hoon Kim
human	WB,IP	Clin Transl Sci; Screening and characterization of myositis-related autoantibodies in COVID-19 patients; Authors - Kai-Fa Teo
human	WB	Nat Commun; Comprehensive proteogenomic characterization of early duodenal cancer reveals the carcinogenesis tracks of different subtypes; Authors - Lingling Li