TARS Monoclonal antibody

TARS Monoclonal Antibody for WB, IF/ICC, ELISA

Cat No. 67828-1-Ig

Clone No. 1A11F10

Publications (1)

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Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

1A11F10, EC:6.1.1.3, TARS1, Threonine tRNA ligase, Threonine--tRNA ligase 1, cytoplasmic

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

CoraLite®594

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Filter: AllWBIHCIF/ICC

WB analysis using 67828-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 67828-1-Ig (TARS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HSC-T6 using 67828-1-Ig
HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67828-1-Ig (TARS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of 4T1 using 67828-1-Ig
4T1 cells were subjected to SDS PAGE followed by western blot with 67828-1-Ig (TARS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of PC-12 using 67828-1-Ig
PC-12 cells were subjected to SDS PAGE followed by western blot with 67828-1-Ig (TARS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse kidney using 67828-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 67828-1-Ig (TARS antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human stomach cancer using 67828-1-Ig
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67828-1-Ig (TARS antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of rat kidney using 67828-1-Ig
Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 67828-1-Ig (TARS antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HeLa using 67828-1-Ig
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using TARS antibody (67828-1-Ig, Clone: 1A11F10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	HeLa cells, HSC-T6 cells, PC-12 cells, 4T1 cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunofluorescence (IF)/ICC	IF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67828-1-Ig targets TARS in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Mouse / IgG2b
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag6614 Product name: Recombinant human TARS protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 394-723 aa of BC000517 Sequence: ENMFSFEVEKELFALKPMNCPGHCLMFDHRPRSWRELPLRLADFGVLHRNELSGALTGLTRVRRFQQDDAHIFCAMEQIEDEIKGCLDFLRTVYSVFGFSFKLNLSTRPEKFLGDIEVWDQAEKQLENSLNEFGEKWELNSGDGAFYGPKIDIQIKDAIGRYHQCATIQLDFQLPIRFNLTYVSHDGDDKKRPVIVHRAILGSVERMIAILTENYGGKWPFWLSPRQVMVVPVGPTCDEYAQKVRQQFHDAKFMADIDLDPGCTLNKKIRNAQLAQYNFILVVGEKEKISGTVNIRTRDNKVHGERTISETIERLQQLKEFRSKQAEEEF Predict reactive species
Full Name	threonyl-tRNA synthetase
Calculated Molecular Weight	83 kDa
Observed Molecular Weight	83-87 kDa
GenBank Accession Number	BC000517
Gene Symbol	TARS
Gene ID (NCBI)	6897
RRID	AB_2918590
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P26639
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TARS (Threonyl-tRNA synthetase), also known as threonine-tRNA ligase or ThrRS, belongs to the aminoacyl-tRNA synthetase family. Aminoacyl-tRNA synthetase (aaRS) catalyzes the specific esterification of an amino acid with the 3'-terminal hydroxyl group of its cognate tRNA. Specificity of aminoacylation catalyzed by aaRSs ensures the accurate translation of the genetic code in the first step of protein synthesis (PMID: 26324710). TARS has some isoforms with the molecular mass of 83, 87 kDa and other MW.

Protocols

Product Specific Protocols
IF protocol for TARS antibody 67828-1-Ig	Download protocol
WB protocol for TARS antibody 67828-1-Ig	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The TARS Monoclonal antibody (1A11F10), catalog number 67828-1-Ig, has a total of 1 citation. It appears in the Journal of Hematology and Oncology (IF: 47.8), in a study on integrated proteogenomic characterization of urothelial carcinoma of the bladder. The associated research field is cancer genomics and urological oncology, with the antibody applied in Western blot for human samples.