TARS Monoclonal antibody

TARS Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

1A11F10

Cat no : 67828-1-Ig

Synonyms

TARS, Threonine tRNA ligase, threonyl tRNA synthetase, ThrRS

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Validation Data Gallery

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  WB analysis using 67828-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67828-1-Ig (TARS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

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  WB analysis of HSC-T6 using 67828-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67828-1-Ig (TARS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of 4T1 using 67828-1-Ig

  4T1 cells were subjected to SDS PAGE followed by western blot with 67828-1-Ig (TARS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-12 using 67828-1-Ig

  PC-12 cells were subjected to SDS PAGE followed by western blot with 67828-1-Ig (TARS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 67828-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using TARS antibody (67828-1-Ig, Clone: 1A11F10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, HSC-T6 cells, PC-12 cells, 4T1 cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

67828-1-Ig targets TARS in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: TARS fusion protein Ag6614
• Full Name: threonyl-tRNA synthetase
• Calculated Molecular Weight: 83 kDa
• Observed Molecular Weight: 83-87 kDa
• GenBank Accession Number: BC000517
• Gene Symbol: TARS
• Gene ID (NCBI): 6897
• RRID: AB_2918590
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TARS (Threonyl-tRNA synthetase), also known as threonine-tRNA ligase or ThrRS, belongs to the aminoacyl-tRNA synthetase family. Aminoacyl-tRNA synthetase (aaRS) catalyzes the specific esterification of an amino acid with the 3'-terminal hydroxyl group of its cognate tRNA. Specificity of aminoacylation catalyzed by aaRSs ensures the accurate translation of the genetic code in the first step of protein synthesis (PMID: 26324710). TARS has some isoforms with the molecular mass of 83, 87 kDa and other MW.

Publications

Species	Application	Title
human	WB	J Hematol Oncol; Integrated proteogenomic characterization of urothelial carcinoma of the bladder.; Authors - Ning Xu