TARSL2 Polyclonal antibody

TARSL2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 24635-1-AP

Synonyms

Threonyl-tRNA synthetase protein 3, Threonyl-tRNA synthetase, Threonine--tRNA ligase 2, cytoplasmic, Threonine  tRNA ligase, TARS3

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Validation Data Gallery

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  WB analysis of L02 using 24635-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 24635-1-AP (TARSL2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 24635-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24635-1-AP (TARSL2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 24635-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24635-1-AP (TARSL2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 24635-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24635-1-AP (TARSL2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of L02 using 24635-1-AP

  Immunofluorescent analysis of L02 cells using 24635-1-AP (TARSL2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: L02 cells, HEK-293 cells
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: L02 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

24635-1-AP targets TARSL2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TARSL2 fusion protein Ag18298
• Full Name: threonyl-tRNA synthetase-like 2
• Calculated Molecular Weight: 802 aa, 93 kDa
• Observed Molecular Weight: 93 kDa
• GenBank Accession Number: BC132671
• Gene Symbol: TARSL2
• Gene ID (NCBI): 123283
• RRID: AB_2879648
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.